Detailed information of TA system TA124209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 4675..5206
• Replicon: plasmid pABRDHK234
• Accession: NZ_CP039699
• Organism: Novosphingobium sp. ABRDHK2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: FA702_RS21600
• Protein ID: WP_004212690.1
• Coordinates: 4675..4956 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: FA702_RS21605
• Protein ID: WP_004212689.1
• Coordinates: 4943..5206 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA702_RS21575	270..817	-	548	Protein_0	IS21-like element helper ATPase IstB	-
FA702_RS23020	814..975	-	162	WP_210417681.1	hypothetical protein	-
FA702_RS21585	1617..2436	-	820	Protein_2	IS21-like element helper ATPase IstB	-
FA702_RS21590	2433..3934	-	1502	Protein_3	IS21 family transposase	-
FA702_RS23025	4145..4603	+	459	WP_210417679.1	hypothetical protein	-
FA702_RS21600	4675..4956	-	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
FA702_RS21605	4943..5206	-	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FA702_RS21610	5590..6027	-	438	WP_007683347.1	metal-binding protein	-
FA702_RS21615	6107..7347	-	1241	Protein_8	MFS transporter	-
FA702_RS21620	7341..8327	-	987	WP_009823975.1	copper resistance protein B	-
FA702_RS21625	8324..10030	-	1707	WP_136958154.1	copper resistance system multicopper oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..210225	210225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T124209 WP_004212690.1 NZ_CP039699:c4956-4675 [Novosphingobium sp. ABRDHK2]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T124209 NZ_CP039699:c4956-4675 [Novosphingobium sp. ABRDHK2]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT124209 WP_004212689.1 NZ_CP039699:c5206-4943 [Novosphingobium sp. ABRDHK2]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT124209 NZ_CP039699:c5206-4943 [Novosphingobium sp. ABRDHK2]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATAGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968