Detailed information of TA system TA124208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 71557..72254
• Replicon: plasmid pABRDHK21
• Accession: NZ_CP039696
• Organism: Novosphingobium sp. ABRDHK2

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: FA702_RS18205
• Protein ID: WP_136957566.1
• Coordinates: 71880..72254 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: T0G937
• Locus tag: FA702_RS18200
• Protein ID: WP_006964370.1
• Coordinates: 71557..71883 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA702_RS18170	67146..67901	+	756	WP_136957561.1	P-type conjugative transfer protein TrbJ	-
FA702_RS18175	67910..68146	+	237	WP_136957562.1	EexN family lipoprotein	-
FA702_RS18180	68157..69881	+	1725	WP_136957563.1	P-type conjugative transfer protein TrbL	-
FA702_RS18185	69884..71002	+	1119	WP_136957564.1	transglycosylase SLT domain-containing protein	-
FA702_RS18190	71023..71322	-	300	WP_136957565.1	hypothetical protein	-
FA702_RS18195	71315..71557	-	243	WP_013041566.1	hypothetical protein	-
FA702_RS18200	71557..71883	-	327	WP_006964370.1	helix-turn-helix domain-containing protein	Antitoxin
FA702_RS18205	71880..72254	-	375	WP_136957566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FA702_RS18210	72681..73037	+	357	WP_136957567.1	sigma-70 family RNA polymerase sigma factor	-
FA702_RS18215	73034..73420	-	387	WP_136957568.1	response regulator	-
FA702_RS18220	73420..73695	-	276	WP_136957569.1	hypothetical protein	-
FA702_RS18225	73692..74693	-	1002	WP_136957509.1	GGDEF domain-containing protein	-
FA702_RS18230	74722..75066	-	345	Protein_91	DDE-type integrase/transposase/recombinase	-
FA702_RS18235	75238..76215	-	978	WP_136957510.1	ExeA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89919	89919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (15-94)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14000.73 Da
• Isoelectric Point: 9.4013

>T124208 WP_136957566.1 NZ_CP039696:c72254-71880 [Novosphingobium sp. ABRDHK2]
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDYQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKTAEEHHRQHQTPRGTA

Nucleotide

• Length: 375 bp

>T124208 NZ_CP039696:c72254-71880 [Novosphingobium sp. ABRDHK2]
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTCGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATAATAGACGACTACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTTGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAAACCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11792.57 Da
• Isoelectric Point: 7.1681

>AT124208 WP_006964370.1 NZ_CP039696:c71883-71557 [Novosphingobium sp. ABRDHK2]
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA

Nucleotide

• Length: 327 bp

>AT124208 NZ_CP039696:c71883-71557 [Novosphingobium sp. ABRDHK2]
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGATCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTGGCCGAGGCCATGCAGGAGCGGAAATTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACTCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTAGCC
ATGCTCACCCGGCTTGGCTGCAAGGTCGATATTGTGGTGCGTCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0J8A803