Detailed information of TA system TA124203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 101217..101815
• Replicon: chromosome
• Accession: NZ_CP039692
• Organism: Agrobacterium larrymoorei strain CFBP5473

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CFBP5473_RS14815
• Protein ID: WP_084631769.1
• Coordinates: 101217..101510 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D7DQ02
• Locus tag: CFBP5473_RS14820
• Protein ID: WP_027676543.1
• Coordinates: 101507..101815 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBP5473_RS14785	96632..97177	+	546	WP_027676548.1	sigma-70 family RNA polymerase sigma factor	-
CFBP5473_RS14790	97174..97878	+	705	WP_027676547.1	anti-sigma factor	-
CFBP5473_RS14795	97946..98764	-	819	WP_027676546.1	alpha/beta hydrolase	-
CFBP5473_RS14800	99275..100495	-	1221	WP_027676545.1	M20/M25/M40 family metallo-hydrolase	-
CFBP5473_RS14805	100542..100862	-	321	Protein_88	ABC transporter ATP-binding protein	-
CFBP5473_RS14810	100836..101147	+	312	WP_084631771.1	UTRA domain-containing protein	-
CFBP5473_RS14815	101217..101510	-	294	WP_084631769.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CFBP5473_RS14820	101507..101815	-	309	WP_027676543.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CFBP5473_RS14825	102101..102953	+	853	Protein_92	manganese catalase family protein	-
CFBP5473_RS14830	103290..104459	-	1170	WP_027676542.1	glutathione-dependent formaldehyde dehydrogenase	-
CFBP5473_RS14835	104694..105578	+	885	WP_027676541.1	PAS domain S-box protein	-
CFBP5473_RS14840	105763..106302	-	540	WP_027676540.1	hypothetical protein	-
CFBP5473_RS14845	106441..106732	+	292	Protein_96	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-57)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10721.37 Da
• Isoelectric Point: 5.8086

>T124203 WP_084631769.1 NZ_CP039692:c101510-101217 [Agrobacterium larrymoorei]
MRLTWSAFALSDRDTIFTFIELENAAAAILVDERIVVAVRRLIDFPASGRAGRIAGTRELVISGTPYVAVYTITEATVRV
LRILHGAQEWPEAFSIS

Nucleotide

• Length: 294 bp

>T124203 NZ_CP039692:c101510-101217 [Agrobacterium larrymoorei]
TTGAGACTAACGTGGTCCGCGTTTGCCTTATCGGATCGCGACACAATTTTTACTTTCATCGAGTTGGAGAACGCGGCCGC
TGCCATTTTGGTCGACGAACGTATCGTCGTTGCAGTCCGTCGCCTGATCGATTTTCCAGCAAGTGGCAGGGCCGGAAGAA
TTGCTGGGACACGTGAACTTGTAATCAGCGGAACGCCTTACGTCGCAGTATATACGATCACAGAAGCTACGGTTCGAGTA
CTGCGTATTTTGCACGGGGCTCAGGAATGGCCTGAAGCTTTTTCAATCAGTTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.53 Da
• Isoelectric Point: 4.8728

>AT124203 WP_027676543.1 NZ_CP039692:c101815-101507 [Agrobacterium larrymoorei]
MAANALVQTRIDADVRDRASLVLEGMGLTVSDAVRILLTRTANEGMLPLELLSGSEAHDTWFRAKVLEALNDTRPDISDD
EVEAHFEKRRHSARNNAAGITS

Nucleotide

• Length: 309 bp

>AT124203 NZ_CP039692:c101815-101507 [Agrobacterium larrymoorei]
ATGGCTGCAAATGCGCTCGTTCAAACTCGTATCGATGCTGACGTTCGTGACCGGGCGTCCCTCGTTCTGGAAGGTATGGG
ACTTACGGTGTCAGACGCGGTTCGCATATTGCTTACGAGGACCGCCAATGAGGGCATGTTGCCGCTTGAACTTCTTTCAG
GCAGCGAGGCTCACGATACCTGGTTTCGTGCAAAGGTGCTTGAGGCGCTAAATGACACAAGACCGGATATTTCCGACGAT
GAAGTGGAAGCCCATTTCGAAAAGCGTCGGCATTCTGCGCGCAATAACGCGGCGGGTATAACGTCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A4D7DQ02