Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pasABC/RelE-HTH
Location 2554085..2554571 Replicon chromosome
Accession NZ_CP039691
Organism Agrobacterium larrymoorei strain CFBP5473

Toxin (Protein)


Gene name pasB Uniprot ID A0A4D7DPW2
Locus tag CFBP5473_RS12430 Protein ID WP_027673882.1
Coordinates 2554302..2554571 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID A0A4D7E236
Locus tag CFBP5473_RS12425 Protein ID WP_027673883.1
Coordinates 2554085..2554318 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBP5473_RS12400 2549409..2549855 + 447 WP_027673887.1 DoxX family protein -
CFBP5473_RS12405 2549907..2550317 - 411 WP_027673886.1 GFA family protein -
CFBP5473_RS12410 2550463..2551161 - 699 WP_027673885.1 ATP phosphoribosyltransferase -
CFBP5473_RS12415 2551158..2552279 - 1122 WP_027673884.1 ATP phosphoribosyltransferase regulatory subunit -
CFBP5473_RS12420 2552288..2553931 - 1644 WP_051441151.1 histidine--tRNA ligase -
CFBP5473_RS12425 2554085..2554318 + 234 WP_027673883.1 ribbon-helix-helix domain-containing protein Antitoxin
CFBP5473_RS12430 2554302..2554571 + 270 WP_027673882.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFBP5473_RS12435 2554575..2555339 + 765 WP_027673881.1 hypothetical protein -
CFBP5473_RS25075 2555582..2555740 + 159 WP_157835785.1 hypothetical protein -
CFBP5473_RS12440 2555792..2556157 + 366 WP_027673879.1 DUF305 domain-containing protein -
CFBP5473_RS12445 2556206..2557120 - 915 WP_027673878.1 LysR family transcriptional regulator -
CFBP5473_RS12450 2557202..2558239 + 1038 WP_027673877.1 putative sulfate exporter family transporter -
CFBP5473_RS12455 2558236..2558658 - 423 WP_027673876.1 hypothetical protein -
CFBP5473_RS12460 2558676..2559305 - 630 WP_027673875.1 DNA-3-methyladenine glycosylase I -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-88)

Antitoxin

Protein of unknown function DUF6290

(6-71)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10564.19 Da        Isoelectric Point: 10.5156

>T124201 WP_027673882.1 NZ_CP039691:2554302-2554571 [Agrobacterium larrymoorei]
MIWEIEFRESARKQLKKLGRQDATRIIAFLSDRISADTNPRRTGQALQGSELGKFWRYRVGDYRIICDIQDQKLVIIAIE
IGHRREVYR

Download         Length: 270 bp

>T124201 NZ_CP039691:2554302-2554571 [Agrobacterium larrymoorei]
TTGATCTGGGAAATTGAATTTCGAGAGTCTGCGCGCAAGCAATTGAAGAAGCTTGGCAGACAGGACGCAACGCGGATCAT
TGCATTTTTGAGTGATCGGATTTCCGCAGACACAAATCCAAGACGAACCGGTCAGGCACTTCAAGGCTCAGAGCTCGGCA
AATTCTGGCGTTATCGCGTCGGTGACTATCGTATTATATGCGACATTCAGGATCAAAAACTTGTCATTATCGCCATCGAA
ATCGGCCACCGCCGGGAAGTATATCGTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 9034.08 Da        Isoelectric Point: 4.7119

>AT124201 WP_027673883.1 NZ_CP039691:2554085-2554318 [Agrobacterium larrymoorei]
MSKQTAIRLPDDTYERLKALSERTGRTSAFYIREAIEKHIEDMEDLYLAEEATRRHIANGERTYSLEEVMRDIDLGN

Download         Length: 234 bp

>AT124201 NZ_CP039691:2554085-2554318 [Agrobacterium larrymoorei]
ATGAGCAAACAAACCGCAATCCGTCTGCCGGATGATACCTATGAGCGGCTGAAGGCGCTTTCAGAGCGCACGGGCCGCAC
CTCCGCCTTTTATATCCGAGAAGCCATCGAAAAGCACATCGAGGATATGGAGGATTTGTATCTGGCGGAAGAAGCGACGC
GGCGACACATTGCAAATGGTGAGCGCACCTATAGTCTTGAAGAAGTGATGCGCGATATTGATCTGGGAAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D7DPW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4D7E236

References