Detailed information of TA system TA124199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2294768..2295354
• Replicon: chromosome
• Accession: NZ_CP039691
• Organism: Agrobacterium larrymoorei strain CFBP5473

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4D7DRV4
• Locus tag: CFBP5473_RS11095
• Protein ID: WP_027677181.1
• Coordinates: 2294768..2295028 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CFBP5473_RS11100
• Protein ID: WP_027677182.1
• Coordinates: 2295025..2295354 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBP5473_RS11070	2290518..2291018	-	501	WP_027677176.1	GNAT family N-acetyltransferase	-
CFBP5473_RS11075	2291103..2291774	-	672	WP_027677177.1	hemolysin III family protein	-
CFBP5473_RS11080	2291892..2292302	-	411	WP_027677178.1	universal stress protein	-
CFBP5473_RS11085	2292533..2293393	+	861	WP_027677179.1	VOC family protein	-
CFBP5473_RS11090	2293498..2294661	+	1164	WP_037171866.1	AI-2E family transporter	-
CFBP5473_RS11095	2294768..2295028	+	261	WP_027677181.1	type II toxin-antitoxin system HicA family toxin	Toxin
CFBP5473_RS11100	2295025..2295354	+	330	WP_027677182.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CFBP5473_RS11105	2295357..2296079	-	723	WP_027677183.1	polysaccharide deacetylase family protein	-
CFBP5473_RS11110	2296134..2297054	-	921	WP_027677184.1	dihydrodipicolinate synthase family protein	-
CFBP5473_RS11115	2297418..2298431	+	1014	WP_136954340.1	IS5 family transposase	-
CFBP5473_RS11120	2298612..2299010	-	399	WP_027677339.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2297418..2298431	1013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (25-84)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9511.90 Da
• Isoelectric Point: 10.4752

>T124199 WP_027677181.1 NZ_CP039691:2294768-2295028 [Agrobacterium larrymoorei]
MKLSKKHRSVLDAVFTDPALAEIDWRDIEALFQACGAEISEGSGSRVRVALNGVRAVFHRPHPQKETDKGAVKSVRRFLS
EAGIRP

Nucleotide

• Length: 261 bp

>T124199 NZ_CP039691:2294768-2295028 [Agrobacterium larrymoorei]
ATGAAGTTGAGCAAGAAGCATCGAAGCGTGCTGGATGCGGTTTTTACCGACCCGGCGCTGGCGGAAATAGACTGGCGAGA
TATCGAAGCCCTTTTCCAGGCCTGCGGCGCGGAAATTTCCGAAGGAAGCGGCTCGCGTGTTCGGGTTGCACTCAACGGGG
TGCGAGCGGTGTTTCACCGTCCGCATCCGCAAAAAGAAACGGACAAGGGAGCAGTGAAATCGGTGCGCCGGTTTCTCAGC
GAAGCAGGAATAAGACCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12265.82 Da
• Isoelectric Point: 4.7809

>AT124199 WP_027677182.1 NZ_CP039691:2295025-2295354 [Agrobacterium larrymoorei]
MTLMHYKGYEAVIEFDEDAEIFHGEVINLRDVITFQGTSAAELKQALAESVEDYISFCKERGEEPEKPFSGQFVVRAEPR
LHKAISTAARRDGVSLNKWVTSTLEKAVS

Nucleotide

• Length: 330 bp

>AT124199 NZ_CP039691:2295025-2295354 [Agrobacterium larrymoorei]
ATGACCTTGATGCATTACAAGGGCTATGAAGCGGTCATCGAGTTCGATGAGGATGCAGAGATTTTCCACGGTGAAGTCAT
CAATCTGCGCGACGTGATCACGTTTCAAGGCACATCCGCAGCGGAGCTGAAACAGGCTTTGGCCGAATCTGTCGAAGACT
ATATCTCCTTCTGCAAGGAGCGTGGGGAAGAACCGGAAAAGCCATTTTCCGGCCAGTTCGTCGTGCGTGCTGAGCCGCGC
CTGCATAAGGCGATTTCAACGGCTGCCCGAAGAGACGGTGTGAGCCTGAATAAGTGGGTGACATCGACGCTGGAAAAGGC
TGTCTCTTGA

Structures

Toxin

• AlphaFold DB: A0A4D7DRV4