Detailed information of TA system TA124195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 828206..828791
• Replicon: chromosome
• Accession: NZ_CP039691
• Organism: Agrobacterium larrymoorei strain CFBP5473

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A4D7DTW6
• Locus tag: CFBP5473_RS03875
• Protein ID: WP_027676625.1
• Coordinates: 828504..828791 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CFBP5473_RS03870
• Protein ID: WP_027676624.1
• Coordinates: 828206..828502 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBP5473_RS03850	824297..825604	-	1308	WP_027676620.1	guanine deaminase	-
CFBP5473_RS03855	825601..826848	-	1248	WP_027676621.1	urate hydroxylase PuuD	-
CFBP5473_RS03860	826963..827856	-	894	WP_027676622.1	transcriptional regulator GcvA	-
CFBP5473_RS03865	827961..828191	+	231	WP_027676623.1	hypothetical protein	-
CFBP5473_RS03870	828206..828502	-	297	WP_027676624.1	putative addiction module antidote protein	Antitoxin
CFBP5473_RS03875	828504..828791	-	288	WP_027676625.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFBP5473_RS03880	828839..829666	-	828	WP_027676626.1	xanthine dehydrogenase accessory protein XdhC	-
CFBP5473_RS03885	829676..832015	-	2340	WP_027676627.1	xanthine dehydrogenase molybdopterin binding subunit	-
CFBP5473_RS03895	832202..833668	-	1467	WP_027676628.1	xanthine dehydrogenase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-89)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11008.78 Da
• Isoelectric Point: 10.5155

>T124195 WP_027676625.1 NZ_CP039691:c828791-828504 [Agrobacterium larrymoorei]
MIRIRKTNIFSDWFTSLRDKRAQARIQVRIDRLSLGLLGDVKFFDGIGEMRIDYGPGYRVYFTRRQNEIVILLCGGDKGS
QTRDIERAIKIAAEV

Nucleotide

• Length: 288 bp

>T124195 NZ_CP039691:c828791-828504 [Agrobacterium larrymoorei]
ATGATCAGAATTCGGAAAACGAATATCTTCTCGGACTGGTTTACGTCCCTGCGAGATAAAAGAGCGCAAGCAAGGATTCA
GGTTCGTATTGACCGTCTTTCGCTGGGACTTCTCGGCGACGTGAAATTTTTCGATGGCATTGGCGAAATGCGGATCGATT
ACGGGCCGGGCTACCGGGTTTATTTCACGCGAAGGCAAAACGAGATAGTTATTCTGCTGTGCGGTGGAGACAAGGGATCT
CAGACGAGAGACATCGAACGCGCCATCAAGATTGCAGCCGAGGTTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10458.02 Da
• Isoelectric Point: 4.8146

>AT124195 WP_027676624.1 NZ_CP039691:c828502-828206 [Agrobacterium larrymoorei]
MKVEVTDWDTSDYLNGNEAVIAYLEASFEDGDPKVIAMALGNIAKAKGMSQVAKQAGITREALYKSLSEKGDPKLSTLIG
VMKALGLRLSVAPEERAA

Nucleotide

• Length: 297 bp

>AT124195 NZ_CP039691:c828502-828206 [Agrobacterium larrymoorei]
ATGAAAGTAGAAGTGACCGATTGGGATACGAGCGATTACCTCAATGGCAATGAAGCCGTGATCGCCTATCTGGAAGCCTC
GTTCGAGGATGGCGATCCGAAGGTCATTGCCATGGCCCTCGGCAACATCGCGAAAGCCAAAGGCATGAGCCAGGTCGCCA
AGCAAGCGGGCATTACCCGGGAAGCGCTTTATAAGTCGCTCAGTGAAAAGGGCGACCCCAAGCTTTCGACCCTCATCGGA
GTGATGAAGGCGTTAGGCTTACGTCTTTCGGTCGCCCCTGAAGAAAGAGCCGCGTAA

Structures

Toxin

• AlphaFold DB: A0A4D7DTW6