Detailed information of TA system TA124191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3091024..3091561
• Replicon: chromosome
• Accession: NZ_CP039690
• Organism: Phreatobacter stygius strain KCTC 52518

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: E8M01_RS14375
• Protein ID: WP_211596725.1
• Coordinates: 3091024..3091281 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: E8M01_RS14380
• Protein ID: WP_136960740.1
• Coordinates: 3091268..3091561 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8M01_RS14360	3086309..3087409	+	1101	WP_136960736.1	LLM class flavin-dependent oxidoreductase	-
E8M01_RS14365	3087539..3088060	+	522	WP_136960737.1	flavin reductase family protein	-
E8M01_RS14370	3088162..3090072	-	1911	WP_136960738.1	sigma-54-dependent Fis family transcriptional regulator	-
E8M01_RS14375	3091024..3091281	+	258	WP_211596725.1	BrnT family toxin	Toxin
E8M01_RS14380	3091268..3091561	+	294	WP_136960740.1	BrnA antitoxin family protein	Antitoxin
E8M01_RS14385	3091605..3091826	-	222	WP_136960741.1	hypothetical protein	-
E8M01_RS14390	3091823..3092038	-	216	WP_136960742.1	hypothetical protein	-
E8M01_RS14395	3092135..3092386	+	252	WP_136960743.1	hypothetical protein	-
E8M01_RS14400	3092450..3093211	-	762	WP_136960744.1	IclR family transcriptional regulator	-
E8M01_RS14405	3093267..3094129	-	863	Protein_2845	CoA ester lyase	-
E8M01_RS14410	3094129..3094587	-	459	WP_136960745.1	MaoC family dehydratase	-
E8M01_RS14415	3094599..3095816	-	1218	WP_136960746.1	CoA transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (23-95)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9382.84 Da
• Isoelectric Point: 9.9914

>T124191 WP_211596725.1 NZ_CP039690:3091024-3091281 [Phreatobacter stygius]
VKITWDEPKRLANLAKHGMDFRVLDGDFFLRSAIVPAKAGRSMAIGRLDDGTIVVVFSRLGTEGISVISMRPASASERRF
LYDEG

Nucleotide

• Length: 258 bp

>T124191 NZ_CP039690:3091024-3091281 [Phreatobacter stygius]
GTGAAAATCACCTGGGATGAACCCAAGCGCCTCGCCAACCTTGCCAAGCACGGCATGGATTTCAGGGTGCTGGACGGTGA
TTTTTTCCTGAGGTCCGCGATCGTGCCGGCCAAAGCCGGCCGGTCCATGGCGATCGGTCGCCTCGACGACGGCACGATCG
TGGTGGTTTTCTCTCGCCTGGGCACTGAAGGCATTTCGGTCATCAGCATGCGTCCGGCGAGCGCGAGCGAGCGGAGGTTT
CTCTATGACGAAGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10740.12 Da
• Isoelectric Point: 10.1968

>AT124191 WP_136960740.1 NZ_CP039690:3091268-3091561 [Phreatobacter stygius]
MTKGKAFKPGQGYSQQDWDEVSDSPELTEAEIRAARPFAEAFPDLAESVRRTRGPQKAPVKRLVSLRLSQDVIDRFKAAG
PGWQSRIDDALRKAAGL

Nucleotide

• Length: 294 bp

>AT124191 NZ_CP039690:3091268-3091561 [Phreatobacter stygius]
ATGACGAAGGGTAAGGCCTTCAAGCCTGGACAGGGCTATTCGCAACAGGACTGGGACGAGGTCTCGGACAGCCCGGAGCT
CACCGAGGCGGAGATCAGGGCGGCGCGACCATTTGCCGAAGCATTTCCGGATCTGGCCGAAAGCGTGCGGCGCACGCGCG
GCCCACAGAAGGCGCCGGTCAAGCGGCTGGTTTCGTTGCGATTGAGCCAGGATGTGATCGACCGTTTCAAGGCTGCCGGG
CCGGGCTGGCAATCGCGCATCGATGACGCCTTGCGCAAGGCGGCGGGGCTCTAG