Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 331422..331954 Replicon plasmid p2
Accession NZ_CP039644
Organism Azospirillum sp. TSA2s

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag E6C67_RS04425 Protein ID WP_136701582.1
Coordinates 331422..331745 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag E6C67_RS04430 Protein ID WP_136701583.1
Coordinates 331742..331954 (-) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6C67_RS04400 326452..327015 + 564 WP_136701577.1 GNAT family N-acetyltransferase -
E6C67_RS04405 327325..328089 + 765 WP_136701578.1 hypothetical protein -
E6C67_RS04410 328082..328996 + 915 WP_136701579.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
E6C67_RS04415 329008..330129 - 1122 WP_136701580.1 Rieske 2Fe-2S domain-containing protein -
E6C67_RS04420 330215..331363 - 1149 WP_136701581.1 glycoside hydrolase -
E6C67_RS04425 331422..331745 - 324 WP_136701582.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E6C67_RS04430 331742..331954 - 213 WP_136701583.1 antitoxin MazE family protein Antitoxin
E6C67_RS04435 332165..333154 + 990 WP_169054788.1 hypothetical protein -
E6C67_RS04440 333171..333362 - 192 WP_136701585.1 hypothetical protein -
E6C67_RS04445 333416..334768 - 1353 WP_136701028.1 IS66 family transposase -
E6C67_RS04450 334862..335638 - 777 WP_136701586.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - gmd / wcbK 1..351631 351631
- flank IS/Tn - - 333416..334768 1352


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-102)

Antitoxin

Antitoxin MazE-like

(8-69)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11622.56 Da        Isoelectric Point: 9.9914

>T124183 WP_136701582.1 NZ_CP039644:c331745-331422 [Azospirillum sp. TSA2s]
MRRGDLVTVALQGDYGKPRPVLVIQSDLFDGHPSVTILPITSALVDAPLFRLSVDPTPGNGLRKRSQVMVDKVQTIARAK
TGEAFGRLEDESMLAVNRALLLWIGLA

Download         Length: 324 bp

>T124183 NZ_CP039644:c331745-331422 [Azospirillum sp. TSA2s]
ATGAGGCGCGGCGATCTGGTGACAGTCGCCTTGCAGGGTGACTACGGCAAGCCGCGTCCGGTACTGGTCATCCAGTCCGA
CCTGTTCGACGGGCATCCCAGCGTCACCATCCTGCCGATCACGAGCGCCTTGGTGGACGCGCCGCTGTTCCGCCTGTCGG
TCGATCCGACTCCGGGCAACGGTCTGCGCAAACGCTCGCAGGTGATGGTCGACAAGGTCCAGACGATCGCCCGCGCCAAG
ACGGGCGAGGCGTTCGGGCGGTTGGAGGATGAAAGCATGCTCGCGGTCAACCGGGCGCTCCTCCTCTGGATCGGGCTGGC
CTGA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 8227.36 Da        Isoelectric Point: 8.0344

>AT124183 WP_136701583.1 NZ_CP039644:c331954-331742 [Azospirillum sp. TSA2s]
MPTPLSFRVQRHRNGLRAAGLRPLQIWVPDTRRPGFAAECRRQSLLVRDTPQEQETMDWLDAVRDIEGWE

Download         Length: 213 bp

>AT124183 NZ_CP039644:c331954-331742 [Azospirillum sp. TSA2s]
ATGCCCACGCCGCTTTCCTTCCGTGTTCAGCGCCATCGCAACGGCCTGCGTGCCGCCGGGCTGCGGCCGTTGCAGATCTG
GGTGCCGGACACCCGCCGGCCTGGCTTCGCCGCCGAATGCCGGCGCCAGTCTCTGCTGGTCCGGGACACGCCGCAGGAGC
AGGAGACGATGGACTGGCTGGACGCGGTTCGTGACATCGAGGGCTGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References