Detailed information of TA system TA124180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 131478..132171
• Replicon: plasmid unnamed
• Accession: NZ_CP039632
• Organism: Pseudomonas veronii strain Pvy

Toxin (Protein)

• Gene name: tad
• Uniprot ID: N2IHR9
• Locus tag: E4167_RS33190
• Protein ID: WP_003151133.1
• Coordinates: 131794..132171 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: E4167_RS33185
• Protein ID: WP_001172026.1
• Coordinates: 131478..131813 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4167_RS33135	126964..127176	-	213	WP_003089113.1	hypothetical protein	-
E4167_RS33140	127184..127459	-	276	WP_003150552.1	mercury resistance system periplasmic binding protein MerP	-
E4167_RS33145	127472..127825	-	354	WP_155678990.1	mercury transporter MerT	-
E4167_RS33150	127901..128299	+	399	WP_003089120.1	Hg(II)-responsive transcriptional regulator	-
E4167_RS33160	128708..129628	-	921	WP_003463565.1	cation diffusion facilitator family transporter	-
E4167_RS33165	129699..130370	-	672	Protein_138	IS30 family transposase	-
E4167_RS33170	130410..130781	-	372	WP_023294894.1	hypothetical protein	-
E4167_RS33175	130778..131104	-	327	WP_000091614.1	hypothetical protein	-
E4167_RS33180	131128..131463	-	336	WP_000741275.1	hypothetical protein	-
E4167_RS33185	131478..131813	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
E4167_RS33190	131794..132171	-	378	WP_003151133.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4167_RS33195	132363..132953	+	591	WP_141123773.1	recombinase family protein	-
E4167_RS33200	132950..135982	+	3033	WP_155678991.1	Tn3 family transposase	-
E4167_RS33205	136015..136377	-	363	Protein_146	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..194607	194607
	-	inside	IScluster/Tn	-	-	122174..138161	15987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.78 Da
• Isoelectric Point: 9.4693

>T124180 WP_003151133.1 NZ_CP039632:c132171-131794 [Pseudomonas veronii]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T124180 NZ_CP039632:c132171-131794 [Pseudomonas veronii]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT124180 WP_001172026.1 NZ_CP039632:c131813-131478 [Pseudomonas veronii]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT124180 NZ_CP039632:c131813-131478 [Pseudomonas veronii]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A024ELN5

Antitoxin

• AlphaFold DB: A0A024EKI7