Detailed information of TA system TA124175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 66580..67273
• Replicon: plasmid pAeca1-b
• Accession: NZ_CP039627
• Organism: Aeromonas caviae strain T25-39

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: C1C91_RS22300
• Protein ID: WP_000182276.1
• Coordinates: 66917..67273 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: C1C91_RS22295
• Protein ID: WP_001172026.1
• Coordinates: 66580..66915 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1C91_RS22260 (C1C91_22500)	61675..63318	-	1644	WP_171277334.1	IS66 family transposase	-
C1C91_RS22265 (C1C91_22505)	63367..63732	-	366	WP_041205730.1	IS66 family insertion sequence element accessory protein TnpB	-
C1C91_RS22270 (C1C91_22510)	63732..64034	-	303	WP_088813953.1	hypothetical protein	-
C1C91_RS22275 (C1C91_22515)	64133..65385	-	1253	Protein_62	HD-GYP domain-containing protein	-
C1C91_RS22280 (C1C91_22520)	65503..65883	-	381	WP_001054412.1	hypothetical protein	-
C1C91_RS22285 (C1C91_22525)	65880..66206	-	327	WP_000091614.1	hypothetical protein	-
C1C91_RS22290 (C1C91_22530)	66230..66565	-	336	WP_000741275.1	hypothetical protein	-
C1C91_RS22295 (C1C91_22535)	66580..66915	-	336	WP_001172026.1	helix-turn-helix transcriptional regulator	Antitoxin
C1C91_RS22300 (C1C91_22540)	66917..67273	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1C91_RS22305 (C1C91_22545)	67465..68067	+	603	WP_010465829.1	recombinase family protein	-
C1C91_RS22310 (C1C91_22550)	68051..71080	+	3030	WP_032432545.1	Tn3 family transposase	-
C1C91_RS22315 (C1C91_22555)	71332..71892	+	561	WP_171277332.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / tet(E) / msr(E) / mph(E)	-	1..221458	221458
	-	inside	IScluster/Tn	aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / tet(E) / msr(E) / mph(E)	-	37215..77357	40142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T124175 WP_000182276.1 NZ_CP039627:c67273-66917 [Aeromonas caviae]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T124175 NZ_CP039627:c67273-66917 [Aeromonas caviae]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT124175 WP_001172026.1 NZ_CP039627:c66915-66580 [Aeromonas caviae]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT124175 NZ_CP039627:c66915-66580 [Aeromonas caviae]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7