Detailed information of TA system TA124104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 4844596..4845181
• Replicon: chromosome
• Accession: NZ_CP039546
• Organism: Methylorubrum populi strain YC-XJ1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: E8E01_RS22880
• Protein ID: WP_141953952.1
• Coordinates: 4844596..4844901 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B1ZLX6
• Locus tag: E8E01_RS22885
• Protein ID: WP_012456643.1
• Coordinates: 4844891..4845181 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8E01_RS22855	4839812..4841218	-	1407	WP_012456637.1	adenosylhomocysteinase	-
E8E01_RS22860	4841417..4841821	-	405	WP_141953949.1	DUF5615 family PIN-like protein	-
E8E01_RS22865	4841802..4842068	-	267	WP_012456639.1	DUF433 domain-containing protein	-
E8E01_RS22870	4842129..4843754	-	1626	WP_141953951.1	AMP-binding protein	-
E8E01_RS22875	4843859..4844470	-	612	WP_012456641.1	nitroreductase family protein	-
E8E01_RS22880	4844596..4844901	+	306	WP_141953952.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E8E01_RS22885	4844891..4845181	+	291	WP_012456643.1	putative addiction module antidote protein	Antitoxin
E8E01_RS22890	4845237..4846034	+	798	WP_141953954.1	enoyl-CoA hydratase	-
E8E01_RS22895	4846114..4846893	+	780	WP_141953956.1	endonuclease/exonuclease/phosphatase family protein	-
E8E01_RS22900	4846784..4848220	-	1437	WP_141953958.1	cardiolipin synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11495.01 Da
• Isoelectric Point: 9.7215

>T124104 WP_141953952.1 NZ_CP039546:4844596-4844901 [Methylorubrum populi]
MPTIRQTRTYSDWFDGLTDRQARARIDVRVRRLSLGNPGDVRHLTGGVSELRIDHGPGYRVYDTQRGQELVILLCGGDKR
RQARDIALALEMVKEVWSDGS

Nucleotide

• Length: 306 bp

>T124104 NZ_CP039546:4844596-4844901 [Methylorubrum populi]
ATGCCGACGATTCGCCAGACCCGAACCTACAGCGACTGGTTCGATGGTTTGACGGATCGGCAGGCCCGAGCCCGGATCGA
TGTGCGTGTGCGCCGTCTTTCGCTCGGTAATCCGGGTGATGTGCGGCATCTGACCGGGGGCGTGTCCGAACTTCGGATCG
ATCACGGACCTGGATATCGGGTTTACGATACGCAGCGGGGCCAGGAGCTCGTCATTCTGCTGTGCGGTGGGGACAAGCGC
CGACAGGCACGCGACATCGCGCTGGCTCTGGAGATGGTGAAGGAGGTTTGGTCTGATGGCTCTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10349.82 Da
• Isoelectric Point: 4.6412

>AT124104 WP_012456643.1 NZ_CP039546:4844891-4845181 [Methylorubrum populi]
MALETKPWDVAEHLQDEEAMAAYIEAVLEDGDPALVAAAIGDIARARGMAQIARETGRSRESLYRALSDRGNPELATLMG
VLKAMGLQLSVRPRTA

Nucleotide

• Length: 291 bp

>AT124104 NZ_CP039546:4844891-4845181 [Methylorubrum populi]
ATGGCTCTTGAGACGAAGCCCTGGGACGTCGCCGAACATCTCCAGGACGAGGAGGCGATGGCTGCCTATATCGAGGCTGT
GCTGGAAGATGGCGACCCGGCCCTTGTCGCTGCCGCAATCGGGGACATCGCCCGTGCCCGGGGCATGGCGCAGATCGCCC
GCGAGACGGGGCGCTCACGCGAGAGTCTGTACCGGGCCCTCTCTGATCGGGGCAATCCGGAACTCGCGACCCTCATGGGT
GTGCTCAAGGCCATGGGGCTGCAACTGAGCGTGCGCCCGCGAACCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A177IRN2