Detailed information of TA system TA124101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 3049761..3050161
• Replicon: chromosome
• Accession: NZ_CP039546
• Organism: Methylorubrum populi strain YC-XJ1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B1ZGP7
• Locus tag: E8E01_RS14305
• Protein ID: WP_012454998.1
• Coordinates: 3049761..3049955 (+)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E8E01_RS14310
• Protein ID: WP_141951831.1
• Coordinates: 3049955..3050161 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8E01_RS14285	3045649..3046761	+	1113	WP_141951828.1	undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase	-
E8E01_RS14290	3046879..3048285	+	1407	WP_012454996.1	UDP-N-acetylmuramate--L-alanine ligase	-
E8E01_RS14295	3048289..3048828	+	540	WP_141951829.1	endonuclease domain-containing protein	-
E8E01_RS14300	3048825..3049754	+	930	WP_141951830.1	UDP-N-acetylmuramate dehydrogenase	-
E8E01_RS14305	3049761..3049955	+	195	WP_012454998.1	type II toxin-antitoxin system HicA family toxin	Toxin
E8E01_RS14310	3049955..3050161	+	207	WP_141951831.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E8E01_RS14315	3050225..3051148	+	924	WP_141951832.1	D-alanine--D-alanine ligase	-
E8E01_RS14320	3051115..3052053	+	939	WP_141951833.1	cell division protein FtsQ/DivIB	-
E8E01_RS14325	3052116..3053438	+	1323	WP_012455002.1	cell division protein FtsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.12 Da
• Isoelectric Point: 10.9929

>T124101 WP_012454998.1 NZ_CP039546:3049761-3049955 [Methylorubrum populi]
MKVREIIRLIETDGWTLVATRGSHRQYKHPTKPGRVTVAGAMSDEVAPGTRGSILKQAGLKGQY

Nucleotide

• Length: 195 bp

>T124101 NZ_CP039546:3049761-3049955 [Methylorubrum populi]
ATGAAGGTCCGCGAGATCATCCGCCTCATCGAGACCGATGGATGGACTCTCGTCGCGACACGGGGCTCCCACCGGCAGTA
CAAGCATCCGACGAAGCCCGGCCGCGTTACGGTGGCCGGGGCGATGTCGGACGAGGTCGCGCCCGGAACGCGGGGGAGCA
TCCTCAAGCAGGCGGGTCTGAAAGGACAGTACTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7325.12 Da
• Isoelectric Point: 4.1947

>AT124101 WP_141951831.1 NZ_CP039546:3049955-3050161 [Methylorubrum populi]
MRYAVVIERAGSNYSAYVPDLPGCIATAATAEAVESEIRDAIRFHIEGLRADGDPVPEPTSRAEYVDA

Nucleotide

• Length: 207 bp

>AT124101 NZ_CP039546:3049955-3050161 [Methylorubrum populi]
ATGCGCTACGCCGTCGTCATCGAACGAGCCGGAAGCAACTACTCGGCCTACGTCCCCGATTTGCCGGGCTGCATCGCCAC
CGCCGCGACGGCTGAAGCCGTCGAAAGCGAGATCCGCGATGCCATCCGCTTCCATATCGAAGGCCTTCGTGCCGACGGCG
ATCCTGTGCCCGAGCCGACGAGCCGGGCCGAATACGTTGATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A177JAT4