Detailed information of TA system TA124098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 792466..793115
• Replicon: chromosome
• Accession: NZ_CP039546
• Organism: Methylorubrum populi strain YC-XJ1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B1Z7Z2
• Locus tag: E8E01_RS03940
• Protein ID: WP_012452719.1
• Coordinates: 792466..792837 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: E8E01_RS03945
• Protein ID: WP_141950404.1
• Coordinates: 792837..793115 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8E01_RS03905	787466..787696	-	231	WP_141950399.1	hypothetical protein	-
E8E01_RS03910	787770..788129	-	360	WP_141954700.1	transcriptional regulator	-
E8E01_RS03915	788129..788704	-	576	WP_141950400.1	hypothetical protein	-
E8E01_RS03920	788888..790039	+	1152	WP_141950401.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
E8E01_RS03925	790045..790905	-	861	WP_141950402.1	C40 family peptidase	-
E8E01_RS03930	791216..791587	+	372	WP_012452717.1	hypothetical protein	-
E8E01_RS03935	791703..792389	-	687	WP_141950403.1	SDR family NAD(P)-dependent oxidoreductase	-
E8E01_RS03940	792466..792837	-	372	WP_012452719.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E8E01_RS03945	792837..793115	-	279	WP_141950404.1	PbsX family transcriptional regulator	Antitoxin
E8E01_RS03950	793172..793888	-	717	WP_041371865.1	MgtC/SapB family protein	-
E8E01_RS03955	794212..795207	+	996	WP_141950405.1	AAA family ATPase	-
E8E01_RS03960	795213..796145	+	933	WP_141950406.1	DUF58 domain-containing protein	-
E8E01_RS03965	796281..796736	+	456	WP_141950407.1	DUF4381 family protein	-
E8E01_RS03970	796742..797761	+	1020	WP_141950408.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-116)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 12878.82 Da
• Isoelectric Point: 8.0423

>T124098 WP_012452719.1 NZ_CP039546:c792837-792466 [Methylorubrum populi]
MGPRPRIPKRGDIYSINPNPVAGREMRDRHYWLVLSPEAVNVHGVAITVVIATVAVGARAAGLAVPISAEGVTGVAVCTQ
LRSFDLRARIANGSASFAGTADIKTVEDVAARVADLLDPPPEV

Nucleotide

• Length: 372 bp

>T124098 NZ_CP039546:c792837-792466 [Methylorubrum populi]
ATGGGACCGCGGCCCCGTATTCCGAAGCGAGGGGACATCTATTCGATCAACCCGAACCCCGTCGCCGGCAGGGAAATGCG
CGATCGGCATTACTGGCTCGTTCTGAGCCCGGAGGCCGTCAACGTGCATGGCGTTGCGATCACCGTGGTGATCGCGACAG
TTGCGGTGGGAGCCCGCGCGGCGGGGCTTGCGGTTCCGATCTCCGCAGAGGGTGTGACGGGGGTGGCGGTATGCACGCAA
TTGCGCAGCTTCGATCTACGTGCCCGTATCGCCAACGGTAGCGCATCGTTTGCGGGGACGGCCGACATCAAAACGGTCGA
GGATGTCGCCGCGCGCGTCGCCGATCTGTTGGATCCTCCGCCCGAGGTTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9512.99 Da
• Isoelectric Point: 9.8281

>AT124098 WP_141950404.1 NZ_CP039546:c793115-792837 [Methylorubrum populi]
MATIVKVRRQGGARIVTLPSALLARIGADAGTSLALDVKGGAIVAKPVADAPAPRRRRYTLSELLVGAEHLPDLYRSVEG
ALDGDPVGNEIG

Nucleotide

• Length: 279 bp

>AT124098 NZ_CP039546:c793115-792837 [Methylorubrum populi]
ATGGCCACCATCGTAAAAGTGCGACGCCAGGGTGGCGCGCGCATCGTCACTCTGCCTTCCGCGCTGTTGGCGCGGATCGG
GGCGGATGCCGGCACGTCTCTGGCTCTCGATGTCAAGGGCGGGGCGATCGTCGCCAAGCCGGTCGCTGACGCTCCGGCTC
CCCGTCGGCGGCGTTACACGCTGTCGGAGCTTCTCGTCGGAGCGGAGCACCTTCCGGATCTCTACCGTAGTGTCGAGGGC
GCTCTCGATGGTGATCCGGTCGGGAACGAGATCGGCTGA

Structures

Toxin

• AlphaFold DB: A0A177IU35