Detailed information of TA system TA124097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 731812..732491
• Replicon: chromosome
• Accession: NZ_CP039546
• Organism: Methylorubrum populi strain YC-XJ1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: E8E01_RS03660
• Protein ID: WP_141950362.1
• Coordinates: 732066..732491 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1Z722
• Locus tag: E8E01_RS03655
• Protein ID: WP_012452661.1
• Coordinates: 731812..732069 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8E01_RS03635	727357..728748	+	1392	WP_141950358.1	hypothetical protein	-
E8E01_RS03640	728745..729290	+	546	WP_141950359.1	hypothetical protein	-
E8E01_RS03645	729337..730641	+	1305	WP_141950360.1	hypothetical protein	-
E8E01_RS03650	730723..731739	+	1017	WP_141950361.1	serine protease	-
E8E01_RS03655	731812..732069	+	258	WP_012452661.1	Arc family DNA-binding protein	Antitoxin
E8E01_RS03660	732066..732491	+	426	WP_141950362.1	type II toxin-antitoxin system VapC family toxin	Toxin
E8E01_RS03665	732617..733468	+	852	WP_141950363.1	OmpA family protein	-
E8E01_RS03670	733515..734477	+	963	WP_186442366.1	hypothetical protein	-
E8E01_RS03675	734546..735535	+	990	WP_141950365.1	hypothetical protein	-
E8E01_RS03680	735789..736052	+	264	WP_141950366.1	hypothetical protein	-
E8E01_RS03685	736115..736690	+	576	WP_141954695.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (9-47)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15129.42 Da
• Isoelectric Point: 5.1333

>T124097 WP_141950362.1 NZ_CP039546:732066-732491 [Methylorubrum populi]
MIVLDTNVISELWRVAPDARAVAWIDAQAIETLYLTAITVAELRFGTATMPPGRRRDIFRDRLENTVLPAFTGRVLPFDL
DASRLYADLMAWARAAGRAIGMADGLIAAAAAVRGFSVATRDTGPFEAAGVGVVNPWQAGV

Nucleotide

• Length: 426 bp

>T124097 NZ_CP039546:732066-732491 [Methylorubrum populi]
ATGATCGTCCTCGACACGAACGTCATCTCGGAGCTGTGGCGGGTCGCCCCCGACGCGCGCGCTGTGGCCTGGATCGATGC
GCAGGCGATCGAAACGCTTTACCTCACGGCGATCACCGTTGCGGAACTCCGCTTCGGCACGGCGACGATGCCGCCGGGCA
GGCGACGGGACATCTTTCGGGATCGGCTGGAGAACACGGTGCTGCCGGCCTTCACCGGCCGCGTGCTGCCCTTCGATCTC
GATGCCTCACGCCTCTACGCCGACCTCATGGCGTGGGCGCGCGCCGCGGGCAGGGCCATCGGCATGGCAGACGGCTTGAT
CGCGGCCGCCGCGGCCGTCCGGGGCTTTTCCGTGGCGACCCGTGACACCGGCCCGTTCGAGGCCGCCGGGGTCGGCGTCG
TCAATCCGTGGCAGGCCGGCGTCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9289.44 Da
• Isoelectric Point: 6.4706

>AT124097 WP_012452661.1 NZ_CP039546:731812-732069 [Methylorubrum populi]
VASITIRNVPEEVHRALRVRATQHGRSTEAEIRAILEQATKPAGRLQLGSLLASIAREAGGLTETEADAFDRLRDTSPAE
PLRFE

Nucleotide

• Length: 258 bp

>AT124097 NZ_CP039546:731812-732069 [Methylorubrum populi]
GTGGCGTCGATCACCATCCGCAACGTTCCCGAAGAGGTGCACCGTGCCCTGCGCGTCCGGGCGACGCAGCATGGGCGCAG
CACCGAGGCCGAGATCCGCGCCATCCTGGAACAGGCGACCAAGCCGGCGGGCCGATTGCAGCTCGGCTCCCTGCTCGCCT
CCATCGCCCGCGAGGCCGGCGGCCTGACCGAGACGGAAGCGGACGCCTTCGACCGGCTTCGCGACACGTCGCCCGCCGAA
CCGCTCCGATTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A177IUB0