Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 217051..217802 Replicon plasmid pHB25-1-vir
Accession NZ_CP039526
Organism Klebsiella pneumoniae subsp. pneumoniae strain HB25-1

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag E8Q43_RS29080 Protein ID WP_014386536.1
Coordinates 217051..217533 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag E8Q43_RS29085 Protein ID WP_004902250.1
Coordinates 217524..217802 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E8Q43_RS29060 213450..214097 - 648 WP_014386537.1 EcsC family protein -
E8Q43_RS29065 214124..214879 - 756 WP_004902235.1 DUF2971 domain-containing protein -
E8Q43_RS29070 214980..215372 - 393 WP_032442757.1 hypothetical protein -
E8Q43_RS29075 215477..216016 - 540 WP_004902239.1 hypothetical protein -
E8Q43_RS29080 217051..217533 - 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
E8Q43_RS29085 217524..217802 - 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
E8Q43_RS29090 217921..218133 - 213 WP_004902255.1 hypothetical protein -
E8Q43_RS29095 218241..218582 - 342 WP_004902257.1 hypothetical protein -
E8Q43_RS29100 219413..219871 - 459 WP_014386535.1 hypothetical protein -
E8Q43_RS29105 220524..220979 - 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
E8Q43_RS29110 221051..221416 + 366 WP_001294656.1 mercuric ion transporter MerT -
E8Q43_RS29115 221432..221707 + 276 WP_000732275.1 mercury resistance system periplasmic binding protein MerP -
E8Q43_RS29120 221735..222160 + 426 WP_000522996.1 organomercurial transporter MerC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 / blaTEM-1B / aac(3)-IId / qacE / aadA2 / dfrA12 / blaSHV-187 / catA1 iucA / iucB / iucC / iucD / iutA / rmpA 1..396373 396373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T124093 WP_014386536.1 NZ_CP039526:c217533-217051 [Klebsiella pneumoniae subsp. pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T124093 NZ_CP039526:c217533-217051 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT124093 WP_004902250.1 NZ_CP039526:c217802-217524 [Klebsiella pneumoniae subsp. pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT124093 NZ_CP039526:c217802-217524 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References