Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 39555..40081 Replicon plasmid pHB25-1-vir
Accession NZ_CP039526
Organism Klebsiella pneumoniae subsp. pneumoniae strain HB25-1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag E8Q43_RS28180 Protein ID WP_000323025.1
Coordinates 39794..40081 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag E8Q43_RS28175 Protein ID WP_000534858.1
Coordinates 39555..39794 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E8Q43_RS28155 35553..36893 + 1341 WP_000589001.1 ISNCY family transposase -
E8Q43_RS28160 37314..37625 + 312 Protein_44 transposase -
E8Q43_RS28170 38876..39292 + 417 Protein_46 cation-efflux pump -
E8Q43_RS28175 39555..39794 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
E8Q43_RS28180 39794..40081 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
E8Q43_RS28185 40153..40311 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
E8Q43_RS30205 40914..41888 + 975 WP_004181899.1 hypothetical protein -
E8Q43_RS28195 42086..42463 - 378 WP_004181901.1 hypothetical protein -
E8Q43_RS28200 43153..44220 + 1068 WP_004181903.1 hypothetical protein -
E8Q43_RS28205 44376..44723 + 348 WP_004181904.1 hypothetical protein -
E8Q43_RS28210 44802..45035 + 234 WP_004181905.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 / blaTEM-1B / aac(3)-IId / qacE / aadA2 / dfrA12 / blaSHV-187 / catA1 iucA / iucB / iucC / iucD / iutA / rmpA 1..396373 396373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T124091 WP_000323025.1 NZ_CP039526:39794-40081 [Klebsiella pneumoniae subsp. pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T124091 NZ_CP039526:39794-40081 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT124091 WP_000534858.1 NZ_CP039526:39555-39794 [Klebsiella pneumoniae subsp. pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT124091 NZ_CP039526:39555-39794 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References