Detailed information of TA system TA124090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 30771..31681
• Replicon: plasmid pHB25-1-vir
• Accession: NZ_CP039526
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain HB25-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: E8Q43_RS28135
• Protein ID: WP_004026354.1
• Coordinates: 31211..31681 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: E8Q43_RS28130
• Protein ID: WP_004181895.1
• Coordinates: 30771..31214 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8Q43_RS28110	26170..26877	+	708	WP_048268734.1	hypothetical protein	-
E8Q43_RS28115	26882..27913	+	1032	WP_029505552.1	hypothetical protein	-
E8Q43_RS28120	28977..29399	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
E8Q43_RS28125	29399..30670	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
E8Q43_RS28130	30771..31214	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
E8Q43_RS28135	31211..31681	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
E8Q43_RS28140	31791..32051	-	261	WP_004026352.1	hypothetical protein	-
E8Q43_RS28145	32622..33803	-	1182	WP_078207741.1	IS481 family transposase	-
E8Q43_RS28150	33946..35478	+	1533	WP_015065592.1	IS3-like element ISKpn38 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 / blaTEM-1B / aac(3)-IId / qacE / aadA2 / dfrA12 / blaSHV-187 / catA1	iucA / iucB / iucC / iucD / iutA / rmpA	1..396373	396373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T124090 WP_004026354.1 NZ_CP039526:31211-31681 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T124090 NZ_CP039526:31211-31681 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT124090 WP_004181895.1 NZ_CP039526:30771-31214 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT124090 NZ_CP039526:30771-31214 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9