124088

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5361192..5361817 Replicon chromosome
Accession NZ_CP039524
Organism Klebsiella pneumoniae subsp. pneumoniae strain HB25-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag E8Q43_RS26945 Protein ID WP_002882817.1
Coordinates 5361192..5361575 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag E8Q43_RS26950 Protein ID WP_004150355.1
Coordinates 5361575..5361817 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E8Q43_RS26930 5358558..5359460 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
E8Q43_RS26935 5359457..5360092 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
E8Q43_RS26940 5360089..5361018 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
E8Q43_RS26945 5361192..5361575 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
E8Q43_RS26950 5361575..5361817 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
E8Q43_RS26955 5362022..5362939 + 918 WP_002882812.1 alpha/beta hydrolase -
E8Q43_RS26960 5362953..5363894 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
E8Q43_RS26965 5363939..5364376 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
E8Q43_RS26970 5364373..5365233 - 861 WP_002882807.1 virulence factor BrkB family protein -
E8Q43_RS26975 5365227..5365826 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T124088 WP_002882817.1 NZ_CP039524:c5361575-5361192 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T124088 NZ_CP039524:c5361575-5361192 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT124088 WP_004150355.1 NZ_CP039524:c5361817-5361575 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT124088 NZ_CP039524:c5361817-5361575 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References