Detailed information of TA system TA124040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 4433..4980
• Replicon: plasmid pCFSAN000189
• Accession: NZ_CP039501
• Organism: Salmonella enterica subsp. enterica serovar Bareilly str. CFSAN000189

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: SEEB0189_RS00045
• Protein ID: WP_252990106.1
• Coordinates: 4702..4980 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A4D6P197
• Locus tag: SEEB0189_RS00040
• Protein ID: WP_000084547.1
• Coordinates: 4433..4702 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SEEB0189_RS00010 (SEEB0189_00010)	437..1168	+	732	WP_020833545.1	type-F conjugative transfer system pilin acetylase TraX	-
SEEB0189_RS00015	1393..1596	+	204	Protein_2	phospholipase D family protein	-
SEEB0189_RS00020	1635..1913	+	279	WP_015633302.1	phospholipase D-like domain-containing protein	-
SEEB0189_RS00025 (SEEB0189_00020)	2022..2288	+	267	WP_001576653.1	replication regulatory protein RepA	-
SEEB0189_RS00030 (SEEB0189_00025)	2538..2615	+	78	WP_001541552.1	RepA leader peptide Tap	-
SEEB0189_RS00035 (SEEB0189_00030)	2596..3477	+	882	WP_023193063.1	incFII family plasmid replication initiator RepA	-
SEEB0189_RS00040 (SEEB0189_00040)	4433..4702	+	270	WP_000084547.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
SEEB0189_RS00045 (SEEB0189_00045)	4702..4980	+	279	WP_252990106.1	type II toxin-antitoxin system toxin YacB	Toxin
SEEB0189_RS00050	4970..5113	-	144	Protein_9	IS110 family transposase	-
SEEB0189_RS00055 (SEEB0189_00050)	5132..5722	-	591	WP_031607984.1	plasmid pRiA4b ORF-3 family protein	-
SEEB0189_RS00060 (SEEB0189_00055)	6968..7477	+	510	WP_131535394.1	hypothetical protein	-
SEEB0189_RS00065 (SEEB0189_00060)	7786..8658	+	873	WP_077915507.1	helix-turn-helix transcriptional regulator	-
SEEB0189_RS00070 (SEEB0189_00065)	8855..9556	+	702	WP_023251326.1	transcriptional activator SprB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..81815	81815

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10566.19 Da
• Isoelectric Point: 9.8636

>T124040 WP_252990106.1 NZ_CP039501:4702-4980 [Salmonella enterica subsp. enterica serovar Bareilly str.]
MEIFWTMLASQDRKRIRGYIAEQNLMAAVAMDQRISDSAGSLAEQPYKGRHGRAEGTRELVIHPHFVLVYEVDKQGGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T124040 NZ_CP039501:4702-4980 [Salmonella enterica subsp. enterica serovar Bareilly str.]
ATGGAGATTTTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGTATCCGCGGGTACATCGCAGAACAAAATCTGATGGC
TGCTGTTGCAATGGACCAACGGATTAGCGATTCAGCCGGCAGCCTGGCTGAACAACCGTATAAAGGACGTCACGGCCGGG
CTGAAGGTACCCGGGAGCTGGTCATCCACCCACATTTTGTTCTCGTTTATGAAGTGGATAAACAGGGAGGAAAAGTTTAT
ATCCTGCGTGTATTACATACTGCGCAGAAGTGGCCATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10250.67 Da
• Isoelectric Point: 8.8270

>AT124040 WP_000084547.1 NZ_CP039501:4433-4702 [Salmonella enterica subsp. enterica serovar Bareilly str.]
MSQVNMSLRIDAELKDAFMAAAKNMDRNGSQLIRDFMRQTVERQHNAWFREQVDAGRQQLQRGEVLPHDMVEASAAAWRD
EMMKKAAGK

Nucleotide

• Length: 270 bp

>AT124040 NZ_CP039501:4433-4702 [Salmonella enterica subsp. enterica serovar Bareilly str.]
ATGTCACAGGTTAATATGAGCTTAAGAATTGATGCCGAACTGAAAGACGCTTTTATGGCTGCAGCAAAAAATATGGACCG
TAACGGCTCCCAGTTAATCCGGGATTTCATGCGCCAGACTGTTGAACGCCAGCATAATGCCTGGTTCCGTGAACAGGTTG
ATGCCGGGCGTCAGCAACTGCAGCGCGGTGAAGTGCTTCCGCATGACATGGTGGAGGCCTCCGCTGCCGCATGGCGTGAT
GAAATGATGAAAAAGGCTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A4D6P197