Detailed information of TA system TA124003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 144663..145402
• Replicon: plasmid pSurvcare220
• Accession: NZ_CP039454
• Organism: Enterobacter bugandensis strain 220

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FBF69_RS23965
• Protein ID: WP_069733745.1
• Coordinates: 144663..145148 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FBF69_RS23970
• Protein ID: WP_069733746.1
• Coordinates: 145136..145402 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF69_RS23945	141672..142043	-	372	WP_048242159.1	hypothetical protein	-
FBF69_RS23950	142086..142553	-	468	WP_048242157.1	hypothetical protein	-
FBF69_RS23955	142553..142822	-	270	WP_136486980.1	hypothetical protein	-
FBF69_RS23960	143054..144451	-	1398	WP_136486982.1	ISNCY family transposase	-
FBF69_RS23965	144663..145148	-	486	WP_069733745.1	GNAT family N-acetyltransferase	Toxin
FBF69_RS23970	145136..145402	-	267	WP_069733746.1	DUF1778 domain-containing protein	Antitoxin
FBF69_RS23980	146056..147069	+	1014	WP_069733748.1	replication initiation protein	-
FBF69_RS23990	147949..148554	+	606	WP_069733750.1	DUF4225 domain-containing protein	-
FBF69_RS23995	148529..148855	-	327	WP_023345530.1	hypothetical protein	-
FBF69_RS24000	148987..149757	-	771	WP_023345531.1	type-F conjugative transfer system pilin acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..151140	151140
	-	flank	IS/Tn	-	-	143054..144451	1397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17762.41 Da
• Isoelectric Point: 8.5240

>T124003 WP_069733745.1 NZ_CP039454:c145148-144663 [Enterobacter bugandensis]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQTLGAARTFVVCKTDTKQVVGFYSLTIGSVNHLEATGSLRRNI
PDPIPVIILARLAVDFSFLGQGLGADLLHDAFLRCYRVAENIGVRAIMVHALTEEAKNFYLHHGFKSSQTQERTLFLKLS
Q

Nucleotide

• Length: 486 bp

>T124003 NZ_CP039454:c145148-144663 [Enterobacter bugandensis]
GTGGGACGTGTAACAGCACCAGAACCGTTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGAGGATTAAAAAACCAGACTCTTGGTGCAGCACGAACATTCGTCGTTTGTAAAACAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGACCATCGGCAGCGTTAACCACCTGGAAGCAACGGGAAGCCTCAGGCGCAATATA
CCAGATCCAATTCCCGTAATCATACTTGCTCGTCTGGCAGTCGATTTTTCATTCCTTGGTCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCATTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGTCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTATCA
CAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10138.54 Da
• Isoelectric Point: 4.4066

>AT124003 WP_069733746.1 NZ_CP039454:c145402-145136 [Enterobacter bugandensis]
MKSDVQLNIRAKESQRALIDAAAEILHKSRTEFILEMACQAAENVILDRRVFNFNEEQYAEFIDMLDAPVSDETAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT124003 NZ_CP039454:c145402-145136 [Enterobacter bugandensis]
ATGAAATCAGATGTTCAGCTCAACATTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGAATTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGGCGGGTATTTAATT
TTAATGAGGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGACGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA