Detailed information of TA system TA124000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3999459..4000079
• Replicon: chromosome
• Accession: NZ_CP039453
• Organism: Enterobacter bugandensis strain 220

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1H8SI38
• Locus tag: FBF69_RS19500
• Protein ID: WP_008499287.1
• Coordinates: 3999861..4000079 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8E1RNX1
• Locus tag: FBF69_RS19495
• Protein ID: WP_028012188.1
• Coordinates: 3999459..3999833 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF69_RS19485	3994587..3995780	+	1194	WP_136486495.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
FBF69_RS19490	3995803..3998949	+	3147	WP_047367620.1	multidrug efflux RND transporter permease subunit AcrB	-
FBF69_RS19495	3999459..3999833	+	375	WP_028012188.1	Hha toxicity modulator TomB	Antitoxin
FBF69_RS19500	3999861..4000079	+	219	WP_008499287.1	hemolysin expression modulator Hha	Toxin
FBF69_RS19505	4000289..4000840	+	552	WP_028012187.1	maltose O-acetyltransferase	-
FBF69_RS19510	4000957..4001424	+	468	WP_028012186.1	membrane protein	-
FBF69_RS19515	4001396..4002856	-	1461	WP_069733270.1	PLP-dependent aminotransferase family protein	-
FBF69_RS19520	4002958..4003668	+	711	WP_136486497.1	GNAT family N-acetyltransferase	-
FBF69_RS19525	4003665..4003805	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
FBF69_RS19530	4003808..4004068	-	261	WP_006176933.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T124000 WP_008499287.1 NZ_CP039453:3999861-4000079 [Enterobacter bugandensis]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T124000 NZ_CP039453:3999861-4000079 [Enterobacter bugandensis]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTTGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGCCTTGCAGAGC
TAACGATGAATAAGCTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.30 Da
• Isoelectric Point: 4.8886

>AT124000 WP_028012188.1 NZ_CP039453:3999459-3999833 [Enterobacter bugandensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT124000 NZ_CP039453:3999459-3999833 [Enterobacter bugandensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAATGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACACTCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1H8SI38