Detailed information of TA system TA123998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 3410247..3411044
• Replicon: chromosome
• Accession: NZ_CP039453
• Organism: Enterobacter bugandensis strain 220

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: FBF69_RS16670
• Protein ID: WP_172616463.1
• Coordinates: 3410247..3410747 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: FBF69_RS16675
• Protein ID: WP_008499829.1
• Coordinates: 3410775..3411044 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF69_RS16640	3405845..3406534	+	690	WP_028012657.1	pyrimidine utilization protein B	-
FBF69_RS16645	3406546..3406932	+	387	WP_008499822.1	pyrimidine utilization protein C	-
FBF69_RS16650	3406940..3407740	+	801	WP_028012656.1	pyrimidine utilization protein D	-
FBF69_RS16655	3407750..3408340	+	591	WP_041910551.1	malonic semialdehyde reductase	-
FBF69_RS16660	3408350..3408844	+	495	WP_028012654.1	pyrimidine utilization flavin reductase protein F	-
FBF69_RS16665	3408866..3410188	+	1323	WP_063437640.1	pyrimidine utilization transport protein G	-
FBF69_RS16670	3410247..3410747	-	501	WP_172616463.1	N-acetyltransferase	Toxin
FBF69_RS16675	3410775..3411044	-	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
FBF69_RS16680	3411106..3412023	-	918	WP_136486169.1	DMT family transporter	-
FBF69_RS16685	3412140..3412310	-	171	WP_001273664.1	general stress protein	-
FBF69_RS16690	3412701..3413297	+	597	WP_041910554.1	NAD(P)H:quinone oxidoreductase	-
FBF69_RS16695	3413316..3413543	+	228	WP_028012649.1	YccJ family protein	-
FBF69_RS16700	3413576..3414817	-	1242	WP_136486171.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
FBF69_RS16705	3414970..3415413	+	444	WP_136486173.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18873.63 Da
• Isoelectric Point: 8.4785

>T123998 WP_172616463.1 NZ_CP039453:c3410747-3410247 [Enterobacter bugandensis]
MFSEGTDYDFGDFDCGEPSLNAFLREHLVRQHNGRILRAYLLKERDHPRVLGYYTLSGSCFERAMLPSKTQQRRIPYLNV
PSVTLGRLAIHKELQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALSEQAKRFYLKLGFIPLAGENSHSLFFPTKAIE
RLFEQA

Nucleotide

• Length: 501 bp

>T123998 NZ_CP039453:c3410747-3410247 [Enterobacter bugandensis]
ATGTTTTCAGAGGGAACCGACTATGATTTCGGGGATTTTGATTGTGGTGAACCCTCGCTAAATGCCTTCCTGAGAGAACA
CCTGGTACGCCAGCACAATGGACGTATTCTACGAGCCTACCTGCTTAAAGAGCGCGACCATCCGCGCGTGCTGGGCTATT
ACACGCTATCGGGAAGTTGCTTTGAGAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGTCGGATCCCTTATCTCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTATTCATAAAGAACTACAGGGAAACGAGTGGGGAACAACGCTTGTTGCACACGC
TATGCGGGTGGTTTACCTGGCGTCGCAGGCTGTGGGAGTACACGGAATATTTGTTGATGCCCTTAGCGAGCAGGCTAAAC
GATTTTATCTGAAGCTGGGGTTCATCCCGCTGGCAGGCGAAAACAGCCATTCTTTGTTCTTCCCGACAAAAGCTATCGAA
CGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT123998 WP_008499829.1 NZ_CP039453:c3411044-3410775 [Enterobacter bugandensis]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT123998 NZ_CP039453:c3411044-3410775 [Enterobacter bugandensis]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATCGAGCAGCACCGTCGTC
TCGTGTTAAACGAAGCCTCCTGGAATGCGGTGATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTCAAGCGC
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43