Detailed information of TA system TA123977

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 2471615..2472347
• Replicon: chromosome
• Accession: NZ_CP039452
• Organism: Enterobacter bugandensis strain 1367

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: FBF68_RS11990
• Protein ID: WP_072051579.1
• Coordinates: 2471615..2471944 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FBF68_RS11995
• Protein ID: WP_045616552.1
• Coordinates: 2471982..2472347 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF68_RS11965	2466821..2467702	-	882	WP_063155574.1	helix-turn-helix domain-containing protein	-
FBF68_RS11970	2467721..2468326	-	606	WP_063155575.1	NAD(P)-binding domain-containing protein	-
FBF68_RS11975	2468434..2469351	+	918	WP_063155576.1	LysR family transcriptional regulator	-
FBF68_RS11980	2469463..2470239	+	777	WP_063155577.1	isocitrate lyase/phosphoenolpyruvate mutase family protein	-
FBF68_RS11985	2470223..2471269	+	1047	WP_045631577.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
FBF68_RS11990	2471615..2471944	-	330	WP_072051579.1	TA system toxin CbtA family protein	Toxin
FBF68_RS11995	2471982..2472347	-	366	WP_045616552.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FBF68_RS12000	2472373..2472594	-	222	WP_062729614.1	DUF987 family protein	-
FBF68_RS12005	2472591..2473133	-	543	WP_045616550.1	DNA repair protein RadC	-
FBF68_RS12010	2473146..2473589	-	444	WP_045616548.1	antirestriction protein	-
FBF68_RS12015	2473620..2474441	-	822	WP_062729616.1	DUF945 domain-containing protein	-
FBF68_RS12020	2474854..2475057	-	204	WP_045616545.1	hypothetical protein	-
FBF68_RS12025	2475104..2475943	-	840	WP_045616543.1	hypothetical protein	-
FBF68_RS12030	2476003..2476437	-	435	WP_045616541.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2469463..2496450	26987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12405.13 Da
• Isoelectric Point: 6.7153

>T123977 WP_072051579.1 NZ_CP039452:c2471944-2471615 [Enterobacter bugandensis]
MQTISSHPTRATQPCLSPVEIWQRLLTYLLLQHYGLTLNDTPFSNDSTIQEHIDAGISLSDAVNFLVEKYGLVRIDRKGF
SWEEQTPYLSVVDIVRARRSTGLLKTDVK

Nucleotide

• Length: 330 bp

>T123977 NZ_CP039452:c2471944-2471615 [Enterobacter bugandensis]
ATGCAAACCATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCTGTTGAAATCTGGCAGCGGCTGCTAAC
TTATCTGTTATTGCAGCACTATGGCCTGACACTGAATGACACACCATTCAGCAATGACTCCACCATCCAGGAACATATTG
ATGCTGGAATATCGCTCAGTGATGCAGTGAACTTTCTGGTAGAAAAATACGGGCTTGTCCGTATCGATCGGAAAGGATTT
TCGTGGGAAGAACAAACCCCGTATCTTTCCGTAGTGGATATTGTGCGAGCAAGACGCTCTACCGGCTTGCTAAAAACTGA
CGTGAAATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13715.51 Da
• Isoelectric Point: 5.1218

>AT123977 WP_045616552.1 NZ_CP039452:c2472347-2471982 [Enterobacter bugandensis]
MDNHSESGTKPESPARQLWGLKRDITPCFGARLVQESNRLHYLADRAEFNGAFTDDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHQHCVTLYHNGLICEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT123977 NZ_CP039452:c2472347-2471982 [Enterobacter bugandensis]
ATGGACAATCACTCAGAATCCGGTACTAAGCCGGAGAGTCCCGCCCGCCAGTTGTGGGGACTGAAACGCGATATAACACC
TTGTTTTGGGGCTCGTCTGGTTCAGGAAAGCAACCGACTGCACTATCTTGCTGACCGAGCAGAATTTAACGGGGCGTTCA
CCGATGATGTTGCAATGCGCCTTGACCAGGCATTTCCGCTCATGATGAAACAACTTGAGCTGATGCTGACCAGTGGAGAA
CTTAACCCTCGCCACCAACACTGCGTTACGCTATACCACAACGGCCTCATCTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTACATTGCTATCTATCCTGAGCAGACTGAGCCGCAATAA