Detailed information of TA system TA123971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 276201..276821
• Replicon: chromosome
• Accession: NZ_CP039452
• Organism: Enterobacter bugandensis strain 1367

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1H8SI38
• Locus tag: FBF68_RS01360
• Protein ID: WP_008499287.1
• Coordinates: 276603..276821 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8E1RNX1
• Locus tag: FBF68_RS01355
• Protein ID: WP_028012188.1
• Coordinates: 276201..276575 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF68_RS01345	271329..272522	+	1194	WP_028012190.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
FBF68_RS01350	272545..275691	+	3147	WP_136431039.1	multidrug efflux RND transporter permease subunit AcrB	-
FBF68_RS01355	276201..276575	+	375	WP_028012188.1	Hha toxicity modulator TomB	Antitoxin
FBF68_RS01360	276603..276821	+	219	WP_008499287.1	hemolysin expression modulator Hha	Toxin
FBF68_RS01365	277031..277582	+	552	WP_028012187.1	maltose O-acetyltransferase	-
FBF68_RS01370	277699..278166	+	468	WP_028012186.1	membrane protein	-
FBF68_RS01375	278138..279598	-	1461	WP_063155350.1	PLP-dependent aminotransferase family protein	-
FBF68_RS01380	279700..280410	+	711	WP_063155351.1	GNAT family N-acetyltransferase	-
FBF68_RS01385	280407..280547	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
FBF68_RS01390	280550..280810	-	261	WP_006176933.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T123971 WP_008499287.1 NZ_CP039452:276603-276821 [Enterobacter bugandensis]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T123971 NZ_CP039452:276603-276821 [Enterobacter bugandensis]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTTGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGCCTGGCAGAGC
TAACGATGAATAAGCTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.30 Da
• Isoelectric Point: 4.8886

>AT123971 WP_028012188.1 NZ_CP039452:276201-276575 [Enterobacter bugandensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT123971 NZ_CP039452:276201-276575 [Enterobacter bugandensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAATGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACACTCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1H8SI38