Detailed information of TA system TA123970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 2705405..2706513
• Replicon: chromosome
• Accession: NZ_CP039448
• Organism: Staphylococcus aureus strain VGC1

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: E8M03_RS13730
• Protein ID: WP_000233000.1
• Coordinates: 2705405..2706274 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: E8M03_RS13735
• Protein ID: WP_000205227.1
• Coordinates: 2706289..2706513 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8M03_RS13695	2700898..2701635	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
E8M03_RS13700	2701760..2701843	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
E8M03_RS13705	2701892..2701975	-	84	Protein_2611	peptide-binding protein	-
E8M03_RS13710	2702385..2703179	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
E8M03_RS13715	2703272..2703752	-	481	Protein_2613	GNAT family N-acetyltransferase	-
E8M03_RS13720	2703749..2704657	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
E8M03_RS13725	2704690..2705424	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
E8M03_RS13730	2705405..2706274	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
E8M03_RS13735	2706289..2706513	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
E8M03_RS13740	2706656..2708218	-	1563	WP_000136908.1	recombinase family protein	-
E8M03_RS13745	2708220..2708636	-	417	WP_000323438.1	recombinase	-
E8M03_RS13750	2708637..2708933	-	297	WP_002360685.1	hypothetical protein	-
E8M03_RS13760	2709445..2710122	-	678	Protein_2621	DNA topoisomerase	-
E8M03_RS13765	2710138..2710740	-	603	WP_001062586.1	recombinase family protein	-
E8M03_RS14750	2710754..2710924	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	erm(B) / aph(3')-III / ant(6)-Ia	-	2700762..2713348	12586
	-	flank	IS/Tn	erm(B) / aph(3')-III / ant(6)-Ia	-	2695982..2704657	8675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T123970 WP_000233000.1 NZ_CP039448:c2706274-2705405 [Staphylococcus aureus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T123970 NZ_CP039448:c2706274-2705405 [Staphylococcus aureus]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT123970 WP_000205227.1 NZ_CP039448:c2706513-2706289 [Staphylococcus aureus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT123970 NZ_CP039448:c2706513-2706289 [Staphylococcus aureus]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1