Detailed information of TA system TA123965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2163150..2163664
• Replicon: chromosome
• Accession: NZ_CP039448
• Organism: Staphylococcus aureus strain VGC1

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: E8M03_RS10920
• Protein ID: WP_000581792.1
• Coordinates: 2163150..2163359 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: E8M03_RS10925
• Protein ID: WP_000138934.1
• Coordinates: 2163371..2163664 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8M03_RS10900	2158658..2160016	-	1359	WP_000611468.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
E8M03_RS10905	2160031..2161101	-	1071	WP_149569700.1	D-alanine--D-alanine ligase	-
E8M03_RS10910	2161419..2162621	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
E8M03_RS10915	2162885..2163022	-	138	WP_000828354.1	hypothetical protein	-
E8M03_RS10920	2163150..2163359	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
E8M03_RS10925	2163371..2163664	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
E8M03_RS10930	2163830..2165314	+	1485	WP_000571549.1	cardiolipin synthase	-
E8M03_RS10935	2165342..2165989	+	648	WP_001187610.1	HD domain-containing protein	-
E8M03_RS10945	2166604..2167476	-	873	WP_000725802.1	membrane protein insertase YidC	-
E8M03_RS10950	2167562..2168203	-	642	WP_000483147.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T123965 WP_000581792.1 NZ_CP039448:c2163359-2163150 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T123965 NZ_CP039448:c2163359-2163150 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT123965 WP_000138934.1 NZ_CP039448:c2163664-2163371 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT123965 NZ_CP039448:c2163664-2163371 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9