Detailed information of TA system TA123964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2143410..2143939
• Replicon: chromosome
• Accession: NZ_CP039448
• Organism: Staphylococcus aureus strain VGC1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E8M03_RS10830
• Protein ID: WP_000621175.1
• Coordinates: 2143410..2143772 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E8M03_RS10835
• Protein ID: WP_000948331.1
• Coordinates: 2143769..2143939 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8M03_RS10810	2140388..2141158	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
E8M03_RS10815	2141133..2141612	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
E8M03_RS10820	2141614..2141940	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E8M03_RS10825	2142059..2143060	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E8M03_RS10830	2143410..2143772	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E8M03_RS10835	2143769..2143939	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E8M03_RS10840	2144024..2145172	-	1149	WP_001280702.1	alanine racemase	-
E8M03_RS10845	2145238..2145597	-	360	WP_000581194.1	holo-ACP synthase	-
E8M03_RS10850	2145601..2146092	-	492	WP_001205915.1	PH domain-containing protein	-
E8M03_RS10855	2146079..2147662	-	1584	WP_001294642.1	PH domain-containing protein	-
E8M03_RS10860	2147655..2148134	-	480	WP_001287083.1	hypothetical protein	-
E8M03_RS10865	2148343..2148903	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T123964 WP_000621175.1 NZ_CP039448:c2143772-2143410 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T123964 NZ_CP039448:c2143772-2143410 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT123964 WP_000948331.1 NZ_CP039448:c2143939-2143769 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT123964 NZ_CP039448:c2143939-2143769 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1