Detailed information of TA system TA123960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 868265..868797
• Replicon: chromosome
• Accession: NZ_CP039448
• Organism: Staphylococcus aureus strain VGC1

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: Q93CD4
• Locus tag: E8M03_RS04215
• Protein ID: WP_001103942.1
• Coordinates: 868477..868797 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: E8M03_RS04210
• Protein ID: WP_001058486.1
• Coordinates: 868265..868474 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8M03_RS04165	863285..864505	-	1221	WP_000266157.1	site-specific integrase	-
E8M03_RS04170	864593..865321	-	729	WP_000733774.1	staphylococcal enterotoxin type K	-
E8M03_RS04175	865345..866073	-	729	WP_001033318.1	staphylococcal enterotoxin type Q	-
E8M03_RS04180	866143..866574	-	432	WP_001228759.1	hypothetical protein	-
E8M03_RS04185	866591..867049	-	459	WP_000281657.1	ImmA/IrrE family metallo-endopeptidase	-
E8M03_RS04190	867061..867393	-	333	WP_001260004.1	helix-turn-helix transcriptional regulator	-
E8M03_RS04195	867586..867849	+	264	WP_000243851.1	helix-turn-helix domain-containing protein	-
E8M03_RS04200	867842..868114	+	273	WP_000091731.1	helix-turn-helix domain-containing protein	-
E8M03_RS04205	868126..868272	+	147	WP_000784875.1	hypothetical protein	-
E8M03_RS04210	868265..868474	+	210	WP_001058486.1	hypothetical protein	Antitoxin
E8M03_RS04215	868477..868797	+	321	WP_001103942.1	DUF1474 family protein	Toxin
E8M03_RS04220	868861..869730	+	870	WP_001002732.1	primase alpha helix C-terminal domain-containing protein	-
E8M03_RS04225	869747..871216	+	1470	WP_000390454.1	virulence-associated E family protein	-
E8M03_RS04235	871502..871864	+	363	WP_001039168.1	hypothetical protein	-
E8M03_RS04240	871866..872150	+	285	WP_000998181.1	hypothetical protein	-
E8M03_RS04245	872147..872788	+	642	WP_001019764.1	hypothetical protein	-
E8M03_RS04250	873305..873646	+	342	WP_001190615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	selk / selq / seb	845250..934685	89435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12694.22 Da
• Isoelectric Point: 4.7419

>T123960 WP_001103942.1 NZ_CP039448:868477-868797 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFSELIQKF
HEIEKASSENFDEESDDAKNSIKVAE

Nucleotide

• Length: 321 bp

>T123960 NZ_CP039448:868477-868797 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCAGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCGTCATCAGAGAACTTTGACGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGAGTA
A

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8252.31 Da
• Isoelectric Point: 4.3718

>AT123960 WP_001058486.1 NZ_CP039448:868265-868474 [Staphylococcus aureus]
MNNEQKEVIEHLVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT123960 NZ_CP039448:868265-868474 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: Q93CD4