123932

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 74390..75043 Replicon chromosome
Accession NZ_CP039434
Organism Enterococcus faecalis strain SGAir0397

Toxin (Protein)


Gene name hicA Uniprot ID R3IHS7
Locus tag E7T04_RS00335 Protein ID WP_002367585.1
Coordinates 74861..75043 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R3IHD2
Locus tag E7T04_RS00330 Protein ID WP_010707936.1
Coordinates 74390..74827 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E7T04_RS00315 69666..70364 + 699 WP_096397454.1 N-acetylmannosamine-6-phosphate 2-epimerase -
E7T04_RS00320 70542..72881 + 2340 WP_137685712.1 alpha-glucosidase -
E7T04_RS00325 73428..74345 + 918 WP_010713649.1 helix-turn-helix domain-containing protein -
E7T04_RS00330 74390..74827 - 438 WP_010707936.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E7T04_RS00335 74861..75043 - 183 WP_002367585.1 type II toxin-antitoxin system HicA family toxin Toxin
E7T04_RS00340 75148..75801 - 654 WP_002356081.1 Crp/Fnr family transcriptional regulator -
E7T04_RS00345 76075..76968 + 894 WP_010775474.1 SDR family oxidoreductase -
E7T04_RS00350 76981..77553 + 573 WP_071646507.1 alkaline shock response membrane anchor protein AmaP -
E7T04_RS00355 77566..77757 + 192 WP_002356087.1 DUF2273 domain-containing protein -
E7T04_RS00360 77770..78282 + 513 WP_002383483.1 Asp23/Gls24 family envelope stress response protein -
E7T04_RS00365 78341..78901 + 561 WP_002356090.1 Asp23/Gls24 family envelope stress response protein -
E7T04_RS00370 78925..79167 + 243 WP_002356092.1 GlsB/YeaQ/YmgE family stress response membrane protein -
E7T04_RS00375 79333..79848 + 516 WP_071646508.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(11-71)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6633.06 Da        Isoelectric Point: 10.8756

>T123932 WP_002367585.1 NZ_CP039434:c75043-74861 [Enterococcus faecalis]
MPMTQKQMVKLLKKHGWLPTDGGKGSHIKMEMSGKRPIIIPHGELKKGTEIGILKEAGLK

Download         Length: 183 bp

>T123932 NZ_CP039434:c75043-74861 [Enterococcus faecalis]
ATGCCAATGACACAAAAACAAATGGTCAAATTGCTAAAAAAGCACGGTTGGTTACCAACCGATGGTGGAAAAGGCTCCCA
TATAAAAATGGAGATGTCTGGCAAAAGACCCATCATTATCCCACACGGAGAATTGAAAAAAGGAACCGAAATAGGGATTT
TAAAAGAGGCAGGGCTGAAATAG

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16267.27 Da        Isoelectric Point: 4.2758

>AT123932 WP_010707936.1 NZ_CP039434:c74827-74390 [Enterococcus faecalis]
MLISYPACFYSVENGYYVYFPDIGGSGTQGDTIPDAIAMASDYLGMMLSDDIEHQRTIHQPSPINTLSLEDNNPFRDDDD
FNFNLADSFVSMVNVELNDYLGTNTLVKKTVTIPKWSDELGKKNKLNFSKTLTDAIVEKSLHVLK

Download         Length: 438 bp

>AT123932 NZ_CP039434:c74827-74390 [Enterococcus faecalis]
ATGCTAATTAGTTATCCTGCATGTTTCTACAGTGTCGAGAATGGTTACTATGTTTATTTCCCAGATATAGGTGGTTCTGG
TACGCAAGGAGATACAATTCCTGATGCAATTGCGATGGCTTCCGATTATTTAGGTATGATGTTATCTGATGATATTGAAC
ATCAAAGAACAATCCACCAGCCATCTCCTATCAATACGTTGTCATTAGAAGATAATAATCCCTTTAGAGATGACGATGAC
TTTAATTTTAACCTTGCAGACTCGTTTGTTTCTATGGTAAATGTAGAACTAAATGATTATTTAGGAACAAATACGTTAGT
CAAAAAAACCGTCACCATTCCCAAGTGGTCTGATGAACTCGGGAAAAAAAATAAATTGAATTTTTCTAAAACCTTGACCG
ATGCCATCGTTGAAAAGTCGCTACATGTTTTAAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3IHS7

Antitoxin

Source ID Structure
AlphaFold DB R3IHD2

References