Detailed information of TA system TA123931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 52112..52737
• Replicon: plasmid unnamed
• Accession: NZ_CP039405
• Organism: Escherichia coli strain 377323_2f

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: E9097_RS25055
• Protein ID: WP_062859959.1
• Coordinates: 52339..52737 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: E9097_RS25050
• Protein ID: WP_000450526.1
• Coordinates: 52112..52339 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E9097_RS25050	52112..52339	+	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin
E9097_RS25055	52339..52737	+	399	WP_062859959.1	tRNA(fMet)-specific endonuclease VapC	Toxin
E9097_RS25060	52746..54899	-	2154	WP_062859960.1	type IV conjugative transfer system coupling protein TraD	-
E9097_RS25065	55220..55913	-	694	Protein_64	hypothetical protein	-
E9097_RS25070	56128..56436	+	309	WP_062859961.1	heavy metal-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	bfpK / bfpJ / bfpI / bfpH / bfpP / bfpF / bfpE / bfpD / bfpU / bfpC / bfpB / bfpG / bfpA	1..85191	85191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14947.18 Da
• Isoelectric Point: 7.8605

>T123931 WP_062859959.1 NZ_CP039405:52339-52737 [Escherichia coli]
MLKFMLDTNICIFTIKNKPTSVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELSRQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T123931 NZ_CP039405:52339-52737 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAGCCCACCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AGCGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTTCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATAGTTGTGACTAACAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT123931 WP_000450526.1 NZ_CP039405:52112-52339 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT123931 NZ_CP039405:52112-52339 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTACCAGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGTA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA