Detailed information of TA system TA123929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 6712..7237
• Replicon: plasmid unnamed
• Accession: NZ_CP039405
• Organism: Escherichia coli strain 377323_2f

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: E9097_RS24755
• Protein ID: WP_001159871.1
• Coordinates: 6712..7017 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: E9097_RS24760
• Protein ID: WP_062860119.1
• Coordinates: 7019..7237 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E9097_RS24715	2047..3069	+	1023	Protein_3	alpha-galactosidase	-
E9097_RS24720	3132..3245	+	114	Protein_4	MFS transporter	-
E9097_RS24725	3240..3539	-	300	Protein_5	integrase core domain-containing protein	-
E9097_RS24730	3538..3993	+	456	Protein_6	IS3 family transposase	-
E9097_RS24735	4026..4568	+	543	Protein_7	IS3-like element ISEc48 family transposase	-
E9097_RS25490	4756..5319	+	564	WP_062860118.1	hypothetical protein	-
E9097_RS24745	5574..6227	-	654	Protein_9	IS3 family transposase	-
E9097_RS25495	6224..6409	+	186	Protein_10	IS91 family transposase	-
E9097_RS24750	6579..6711	-	133	Protein_11	phage integrase family protein	-
E9097_RS24755	6712..7017	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
E9097_RS24760	7019..7237	-	219	WP_062860119.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
E9097_RS24770	7833..8077	+	245	Protein_14	hypothetical protein	-
E9097_RS24775	8077..8667	+	591	WP_062860120.1	hypothetical protein	-
E9097_RS25405	8792..9655	-	864	WP_062860121.1	IS3 family transposase	-
E9097_RS25410	9631..10137	-	507	WP_062897179.1	helix-turn-helix domain-containing protein	-
E9097_RS24785	10443..11258	+	816	WP_062860059.1	helix-turn-helix transcriptional regulator	-
E9097_RS24790	11498..11635	-	138	WP_062860058.1	hypothetical protein	-
E9097_RS24795	11646..11777	-	132	WP_062860057.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	bfpK / bfpJ / bfpI / bfpH / bfpP / bfpF / bfpE / bfpD / bfpU / bfpC / bfpB / bfpG / bfpA	1..85191	85191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T123929 WP_001159871.1 NZ_CP039405:c7017-6712 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T123929 NZ_CP039405:c7017-6712 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8402.37 Da
• Isoelectric Point: 4.5203

>AT123929 WP_062860119.1 NZ_CP039405:c7237-7019 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVAWFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT123929 NZ_CP039405:c7237-7019 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCTGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8