Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4613066..4613697 Replicon chromosome
Accession NZ_CP039404
Organism Escherichia coli strain 377323_2f

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag E9097_RS23290 Protein ID WP_001260301.1
Coordinates 4613422..4613697 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E9097_RS23285 Protein ID WP_000593555.1
Coordinates 4613066..4613425 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E9097_RS23260 4609198..4610142 + 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
E9097_RS23265 4610139..4610972 + 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
E9097_RS23270 4610972..4611736 + 765 WP_001136232.1 nickel import ATP-binding protein NikD -
E9097_RS23275 4611733..4612539 + 807 WP_000173679.1 nickel import ATP-binding protein NikE -
E9097_RS23280 4612545..4612946 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
E9097_RS23285 4613066..4613425 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E9097_RS23290 4613422..4613697 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
E9097_RS23295 4613770..4614894 - 1125 WP_024231700.1 ABC transporter permease -
E9097_RS23300 4614894..4617629 - 2736 WP_024231701.1 ribosome-associated ATPase/putative transporter RbbA -
E9097_RS23305 4617626..4618693 - 1068 WP_024231702.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T123927 WP_001260301.1 NZ_CP039404:c4613697-4613422 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T123927 NZ_CP039404:c4613697-4613422 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT123927 WP_000593555.1 NZ_CP039404:c4613425-4613066 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT123927 NZ_CP039404:c4613425-4613066 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References