Detailed information of TA system TA123926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4570478..4571073
• Replicon: chromosome
• Accession: NZ_CP039404
• Organism: Escherichia coli strain 377323_2f

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A826P6U8
• Locus tag: E9097_RS23045
• Protein ID: WP_000155160.1
• Coordinates: 4570478..4570855 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A827G8B6
• Locus tag: E9097_RS23050
• Protein ID: WP_000557316.1
• Coordinates: 4570852..4571073 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E9097_RS23025	4566054..4567124	-	1071	WP_001357972.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-
E9097_RS23030	4567126..4567971	-	846	WP_000572183.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
E9097_RS23035	4567968..4568855	-	888	WP_000099282.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
E9097_RS23040	4568953..4570269	-	1317	WP_000803589.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
E9097_RS23045	4570478..4570855	-	378	WP_000155160.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
E9097_RS23050	4570852..4571073	-	222	WP_000557316.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E9097_RS23055	4571161..4571874	-	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
E9097_RS23060	4571876..4572643	-	768	WP_000084170.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
E9097_RS23065	4572640..4573917	-	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
E9097_RS23070	4573914..4574840	-	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
E9097_RS23075	4574888..4575997	-	1110	WP_001297972.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4565314..4573917	8603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13945.20 Da
• Isoelectric Point: 6.4830

>T123926 WP_000155160.1 NZ_CP039404:c4570855-4570478 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGLILAANPEFVELTVDVAAGKVSLEDIVTRLRQHGT

Nucleotide

• Length: 378 bp

>T123926 NZ_CP039404:c4570855-4570478 [Escherichia coli]
ATGACCATTCAGTTTATCTCTGCGGAGGAGATAATTTATTTCCACGATAAGTTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTACGAGTATGAAGGCGTTTCCGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATTTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCCTCATACTTGCTGCAAACCCAGAGTTTGTGGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACACGGAACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8106.25 Da
• Isoelectric Point: 5.5759

>AT123926 WP_000557316.1 NZ_CP039404:c4571073-4570852 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIMELADK

Nucleotide

• Length: 222 bp

>AT123926 NZ_CP039404:c4571073-4570852 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGACAGAACCTTGCATTGGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCCGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CGGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTATGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A826P6U8

Antitoxin

• AlphaFold DB: A0A827G8B6