Detailed information of TA system TA123925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4528087..4528841
• Replicon: chromosome
• Accession: NZ_CP039404
• Organism: Escherichia coli strain 377323_2f

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A822TTG1
• Locus tag: E9097_RS22855
• Protein ID: WP_021560068.1
• Coordinates: 4528356..4528841 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: E9097_RS22850
• Protein ID: WP_000801915.1
• Coordinates: 4528087..4528365 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E9097_RS22845	4525315..4528020	+	2706	WP_000906938.1	HTH-type transcriptional regulator MalT	-
E9097_RS22850	4528087..4528365	+	279	WP_000801915.1	DUF1778 domain-containing protein	Antitoxin
E9097_RS22855	4528356..4528841	+	486	WP_021560068.1	GNAT family N-acetyltransferase	Toxin
E9097_RS22860	4528891..4529919	-	1029	WP_024231689.1	RNA 3'-terminal phosphate cyclase	-
E9097_RS22865	4529923..4531149	-	1227	WP_001105471.1	RNA-splicing ligase RtcB	-
E9097_RS22870	4531368..4531982	+	615	Protein_4379	DNA-binding transcriptional regulator RtcR	-
E9097_RS22875	4531964..4532722	-	759	WP_001296480.1	DeoR/GlpR family transcriptional regulator	-
E9097_RS22880	4532739..4533569	-	831	WP_000928734.1	rhomboid family intramembrane serine protease GlpG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17680.60 Da
• Isoelectric Point: 9.4066

>T123925 WP_021560068.1 NZ_CP039404:4528356-4528841 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSSLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T123925 NZ_CP039404:4528356-4528841 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCAGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGCTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGGGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10450.97 Da
• Isoelectric Point: 4.6336

>AT123925 WP_000801915.1 NZ_CP039404:4528087-4528365 [Escherichia coli]
MKPESKETPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT123925 NZ_CP039404:4528087-4528365 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAACACCAATCAATATCAGGGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTGGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A822TTG1