Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3874023..3874606 Replicon chromosome
Accession NZ_CP039404
Organism Escherichia coli strain 377323_2f

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag E9097_RS19675 Protein ID WP_024232025.1
Coordinates 3874023..3874358 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag E9097_RS19680 Protein ID WP_000581937.1
Coordinates 3874358..3874606 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E9097_RS19660 3869909..3871207 - 1299 WP_000036723.1 phosphopyruvate hydratase -
E9097_RS19665 3871295..3872932 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
E9097_RS19670 3873160..3873951 - 792 WP_001071670.1 nucleoside triphosphate pyrophosphohydrolase -
E9097_RS19675 3874023..3874358 - 336 WP_024232025.1 endoribonuclease MazF Toxin
E9097_RS19680 3874358..3874606 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
E9097_RS19685 3874684..3876918 - 2235 WP_000226815.1 GTP pyrophosphokinase -
E9097_RS19690 3876966..3878267 - 1302 WP_062860025.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 11968.82 Da        Isoelectric Point: 7.7127

>T123923 WP_024232025.1 NZ_CP039404:c3874358-3874023 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWGARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T123923 NZ_CP039404:c3874358-3874023 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGGGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT123923 WP_000581937.1 NZ_CP039404:c3874606-3874358 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT123923 NZ_CP039404:c3874606-3874358 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References