Detailed information of TA system TA123907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 955421..955971
• Replicon: chromosome
• Accession: NZ_CP039404
• Organism: Escherichia coli strain 377323_2f

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: E9097_RS04840
• Protein ID: WP_062859912.1
• Coordinates: 955657..955971 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: E9097_RS04835
• Protein ID: WP_000125566.1
• Coordinates: 955421..955654 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E9097_RS04810	950494..950781	+	288	WP_001682556.1	ferredoxin-like protein FixX	-
E9097_RS04815	950840..952171	+	1332	WP_001183207.1	MFS transporter	-
E9097_RS04820	952279..952809	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
E9097_RS04825	952802..954664	+	1863	WP_000377123.1	glutathione-regulated potassium-efflux system protein KefC	-
E9097_RS04830	954856..955335	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
E9097_RS04835	955421..955654	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
E9097_RS04840	955657..955971	+	315	WP_062859912.1	CcdB family protein	Toxin
E9097_RS04845	955968..956816	-	849	WP_000257182.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
E9097_RS04850	956823..957200	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
E9097_RS04855	957203..958024	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
E9097_RS04860	958021..959010	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
E9097_RS04865	959010..960296	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11699.67 Da
• Isoelectric Point: 9.0851

>T123907 WP_062859912.1 NZ_CP039404:955657-955971 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGT

Nucleotide

• Length: 315 bp

>T123907 NZ_CP039404:955657-955971 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGACTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT123907 WP_000125566.1 NZ_CP039404:955421-955654 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT123907 NZ_CP039404:955421-955654 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9