Detailed information of TA system TA123900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4371559..4372154
• Replicon: chromosome
• Accession: NZ_CP039403
• Organism: Escherichia coli strain 382634_2f

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A826P6U8
• Locus tag: E4311_RS21585
• Protein ID: WP_000155160.1
• Coordinates: 4371559..4371936 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A827G8B6
• Locus tag: E4311_RS21590
• Protein ID: WP_000557316.1
• Coordinates: 4371933..4372154 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4311_RS21565	4367135..4368205	-	1071	WP_001357972.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-
E4311_RS21570	4368207..4369052	-	846	WP_000572183.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
E4311_RS21575	4369049..4369936	-	888	WP_000099282.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
E4311_RS21580	4370034..4371350	-	1317	WP_000803589.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
E4311_RS21585	4371559..4371936	-	378	WP_000155160.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
E4311_RS21590	4371933..4372154	-	222	WP_000557316.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E4311_RS21595	4372242..4372955	-	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
E4311_RS21600	4372957..4373724	-	768	WP_000084170.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
E4311_RS21605	4373721..4374998	-	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
E4311_RS21610	4374995..4375921	-	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
E4311_RS21615	4375969..4377078	-	1110	WP_001297972.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4366395..4374998	8603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13945.20 Da
• Isoelectric Point: 6.4830

>T123900 WP_000155160.1 NZ_CP039403:c4371936-4371559 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGLILAANPEFVELTVDVAAGKVSLEDIVTRLRQHGT

Nucleotide

• Length: 378 bp

>T123900 NZ_CP039403:c4371936-4371559 [Escherichia coli]
ATGACCATTCAGTTTATCTCTGCGGAGGAGATAATTTATTTCCACGATAAGTTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTACGAGTATGAAGGCGTTTCCGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATTTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCCTCATACTTGCTGCAAACCCAGAGTTTGTGGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACACGGAACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8106.25 Da
• Isoelectric Point: 5.5759

>AT123900 WP_000557316.1 NZ_CP039403:c4372154-4371933 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIMELADK

Nucleotide

• Length: 222 bp

>AT123900 NZ_CP039403:c4372154-4371933 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGACAGAACCTTGCATTGGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCCGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CGGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTATGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A826P6U8

Antitoxin

• AlphaFold DB: A0A827G8B6