Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3663879..3664462 Replicon chromosome
Accession NZ_CP039403
Organism Escherichia coli strain 382634_2f

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag E4311_RS18155 Protein ID WP_024232025.1
Coordinates 3663879..3664214 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag E4311_RS18160 Protein ID WP_000581937.1
Coordinates 3664214..3664462 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4311_RS18140 3659765..3661063 - 1299 WP_000036723.1 phosphopyruvate hydratase -
E4311_RS18145 3661151..3662788 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
E4311_RS18150 3663016..3663807 - 792 WP_001071670.1 nucleoside triphosphate pyrophosphohydrolase -
E4311_RS18155 3663879..3664214 - 336 WP_024232025.1 endoribonuclease MazF Toxin
E4311_RS18160 3664214..3664462 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
E4311_RS18165 3664540..3666774 - 2235 WP_000226815.1 GTP pyrophosphokinase -
E4311_RS18170 3666822..3668123 - 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 11968.82 Da        Isoelectric Point: 7.7127

>T123897 WP_024232025.1 NZ_CP039403:c3664214-3663879 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWGARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T123897 NZ_CP039403:c3664214-3663879 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGGGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT123897 WP_000581937.1 NZ_CP039403:c3664462-3664214 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT123897 NZ_CP039403:c3664462-3664214 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References