Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1269596..1270214 Replicon chromosome
Accession NZ_CP039403
Organism Escherichia coli strain 382634_2f

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E4311_RS06100 Protein ID WP_001291435.1
Coordinates 1269596..1269814 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E4311_RS06105 Protein ID WP_000344800.1
Coordinates 1269840..1270214 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4311_RS06070 1265486..1265797 - 312 WP_000409908.1 MGMT family protein -
E4311_RS06080 1266176..1266529 + 354 WP_000878140.1 DUF1428 domain-containing protein -
E4311_RS06085 1266571..1268121 - 1551 WP_001357014.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E4311_RS06090 1268285..1268755 - 471 WP_000136192.1 YlaC family protein -
E4311_RS06095 1268871..1269422 - 552 WP_000102539.1 maltose O-acetyltransferase -
E4311_RS06100 1269596..1269814 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E4311_RS06105 1269840..1270214 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E4311_RS06110 1270759..1273908 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
E4311_RS06115 1273931..1275124 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1264087..1265460 1373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T123882 WP_001291435.1 NZ_CP039403:c1269814-1269596 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T123882 NZ_CP039403:c1269814-1269596 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT123882 WP_000344800.1 NZ_CP039403:c1270214-1269840 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT123882 NZ_CP039403:c1270214-1269840 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References