Detailed information of TA system TA123877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 66261..67064
• Replicon: plasmid unnamed4
• Accession: NZ_CP039392
• Organism: Enterobacter hormaechei strain cre64

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A7W3E3C9
• Locus tag: EZJ11_RS27080
• Protein ID: WP_022652313.1
• Coordinates: 66534..67064 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: EZJ11_RS27075
• Protein ID: WP_022652312.1
• Coordinates: 66261..66530 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZJ11_RS27060 (EZJ11_27430)	62566..62873	-	308	Protein_74	IS1-like element transposase	-
EZJ11_RS27065 (EZJ11_27440)	63350..64090	+	741	WP_022652310.1	site-specific integrase	-
EZJ11_RS27070 (EZJ11_27445)	64416..65405	-	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
EZJ11_RS27075 (EZJ11_27450)	66261..66530	+	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
EZJ11_RS27080 (EZJ11_27455)	66534..67064	+	531	WP_022652313.1	GNAT family N-acetyltransferase	Toxin
EZJ11_RS27085 (EZJ11_27460)	67232..68089	+	858	WP_218969170.1	IS5 family transposase	-
EZJ11_RS27090 (EZJ11_27465)	68290..69126	-	837	Protein_80	Tn3 family transposase	-
EZJ11_RS27095 (EZJ11_27470)	69404..70408	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
EZJ11_RS27100	70473..71036	-	564	WP_181959262.1	cytochrome C	-
EZJ11_RS27105 (EZJ11_27475)	71035..72015	+	981	WP_104868957.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	mrkA / mrkB / mrkC / mrkD / mrkF / mrkA / mrkB / mrkC / mrkD / mrkF	1..118167	118167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20349.26 Da
• Isoelectric Point: 6.7496

>T123877 WP_022652313.1 NZ_CP039392:66534-67064 [Enterobacter hormaechei]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T123877 NZ_CP039392:66534-67064 [Enterobacter hormaechei]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT123877 WP_022652312.1 NZ_CP039392:66261-66530 [Enterobacter hormaechei]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT123877 NZ_CP039392:66261-66530 [Enterobacter hormaechei]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3E3C9

Antitoxin

• AlphaFold DB: A0A2Z3XDS5