Detailed information of TA system TA123873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 40544..41055
• Replicon: plasmid unnamed1
• Accession: NZ_CP039389
• Organism: Enterobacter hormaechei strain cre64

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W0NIW1
• Locus tag: EZJ11_RS24085
• Protein ID: WP_020956903.1
• Coordinates: 40544..40819 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EZJ11_RS24090
• Protein ID: WP_020956904.1
• Coordinates: 40831..41055 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZJ11_RS24055 (EZJ11_24300)	36581..36868	-	288	WP_020956899.1	TrbC/VirB2 family protein	-
EZJ11_RS24060 (EZJ11_24305)	36855..37493	-	639	WP_020956900.1	transglycosylase SLT domain-containing protein	-
EZJ11_RS24065 (EZJ11_24310)	37706..38038	+	333	WP_227551116.1	helix-turn-helix transcriptional regulator	-
EZJ11_RS24070 (EZJ11_24315)	38068..39258	-	1191	WP_041937886.1	MobP1 family relaxase	-
EZJ11_RS24075 (EZJ11_24320)	39260..39814	-	555	WP_020956901.1	DNA distortion polypeptide 1	-
EZJ11_RS24080 (EZJ11_24325)	40256..40522	-	267	WP_020956902.1	hypothetical protein	-
EZJ11_RS24085 (EZJ11_24330)	40544..40819	-	276	WP_020956903.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
EZJ11_RS24090 (EZJ11_24335)	40831..41055	-	225	WP_020956904.1	DUF6290 family protein	Antitoxin
EZJ11_RS24095 (EZJ11_24340)	41359..41607	-	249	WP_020956905.1	hypothetical protein	-
EZJ11_RS24100 (EZJ11_24345)	41665..41877	-	213	WP_020956906.1	hypothetical protein	-
EZJ11_RS24105	42261..42542	-	282	WP_088912116.1	hypothetical protein	-
EZJ11_RS24110 (EZJ11_24350)	42547..42768	-	222	WP_020956907.1	helix-turn-helix domain-containing protein	-
EZJ11_RS24115 (EZJ11_24355)	42765..43010	-	246	WP_020956908.1	hypothetical protein	-
EZJ11_RS24120 (EZJ11_24360)	43007..43549	-	543	WP_020956909.1	hypothetical protein	-
EZJ11_RS24125 (EZJ11_24365)	43640..44164	-	525	WP_020956910.1	DnaJ domain-containing protein	-
EZJ11_RS24130	44272..44631	+	360	WP_020956911.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49917	49917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-88)

Antitoxin

• Protein of unknown function DUF6290 (6-72)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10922.82 Da
• Isoelectric Point: 10.6500

>T123873 WP_020956903.1 NZ_CP039389:c40819-40544 [Enterobacter hormaechei]
MTRYRTEYTKRFVKEFKKIDTVHQRVIQKYIEAHINNALNPRLTGKILKGDKAEFIRWRIGDYRLIATIEDDKLLVIALS
VGHRKDVYKKF

Nucleotide

• Length: 276 bp

>T123873 NZ_CP039389:c40819-40544 [Enterobacter hormaechei]
ATGACACGCTACCGGACAGAATACACAAAACGGTTCGTCAAAGAGTTTAAGAAAATTGACACGGTACACCAGAGGGTAAT
TCAGAAGTACATTGAGGCGCACATCAACAACGCACTCAATCCGCGCTTGACGGGTAAAATCCTGAAAGGAGATAAAGCCG
AATTTATCCGCTGGCGTATCGGTGATTACCGTCTGATTGCCACTATTGAAGACGATAAATTGCTGGTCATTGCGTTAAGC
GTGGGACACCGCAAAGACGTGTACAAAAAGTTTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8535.52 Da
• Isoelectric Point: 4.3313

>AT123873 WP_020956904.1 NZ_CP039389:c41055-40831 [Enterobacter hormaechei]
MNKNMTKAIRLNDEDFNLFESYANAKGITFSELCKSAVREKIEDELDLQCANESYADYLSDKTTISHDELFKSM

Nucleotide

• Length: 225 bp

>AT123873 NZ_CP039389:c41055-40831 [Enterobacter hormaechei]
ATGAACAAAAACATGACGAAAGCGATCCGGTTGAATGACGAAGATTTTAACCTGTTTGAGTCTTACGCCAACGCAAAAGG
GATAACCTTCAGCGAATTGTGCAAAAGCGCAGTACGGGAAAAAATCGAGGACGAATTAGATTTGCAATGTGCTAATGAAA
GCTACGCGGATTATCTCAGCGACAAAACGACAATCAGCCACGACGAACTGTTTAAATCTATGTGA

Structures

Toxin

• AlphaFold DB: A0A384ZT31