Detailed information of TA system TA123871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4606994..4607599
• Replicon: chromosome
• Accession: NZ_CP039388
• Organism: Enterobacter hormaechei strain cre64

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: EZJ11_RS22335
• Protein ID: WP_012906750.1
• Coordinates: 4606994..4607173 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EZJ11_RS22340
• Protein ID: WP_250351808.1
• Coordinates: 4607195..4607599 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZJ11_RS22305 (EZJ11_22600)	4602323..4603039	+	717	WP_032619512.1	ABC transporter ATP-binding protein	-
EZJ11_RS22310 (EZJ11_22605)	4603205..4603675	+	471	WP_017384432.1	hypothetical protein	-
EZJ11_RS22315 (EZJ11_22610)	4603681..4604607	-	927	WP_032619511.1	LysR substrate-binding domain-containing protein	-
EZJ11_RS22320 (EZJ11_22615)	4604637..4604960	-	324	WP_017384434.1	hypothetical protein	-
EZJ11_RS22325	4604963..4605355	-	393	Protein_4365	DUF4225 domain-containing protein	-
EZJ11_RS22330	4605377..4605718	-	342	WP_223862348.1	DUF4225 domain-containing protein	-
EZJ11_RS22335 (EZJ11_22625)	4606994..4607173	+	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
EZJ11_RS22340 (EZJ11_22630)	4607195..4607599	+	405	WP_250351808.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EZJ11_RS22345 (EZJ11_22635)	4607640..4608710	-	1071	WP_032635096.1	glycosyltransferase family 9 protein	-
EZJ11_RS22350 (EZJ11_22640)	4608787..4609365	-	579	WP_032619505.1	tail fiber assembly protein	-
EZJ11_RS22355 (EZJ11_22645)	4609365..4611542	-	2178	WP_058670599.1	phage tail protein	-
EZJ11_RS22360 (EZJ11_22650)	4611545..4612096	-	552	WP_032619504.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4602335..4645870	43535

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T123871 WP_012906750.1 NZ_CP039388:4606994-4607173 [Enterobacter hormaechei]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T123871 NZ_CP039388:4606994-4607173 [Enterobacter hormaechei]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14648.47 Da
• Isoelectric Point: 4.2136

>AT123871 WP_250351808.1 NZ_CP039388:4607195-4607599 [Enterobacter hormaechei]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGEDYQDAIESVREAIEAHIELLAEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVINQR

Nucleotide

• Length: 405 bp

>AT123871 NZ_CP039388:4607195-4607599 [Enterobacter hormaechei]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGAAGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGCTGAAGATGGCG
AGAGCGTTCCCGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAGTACGCCGGTGCGGTATGGGCCCTAGTTGAC
GTGGATATAACACGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTACCCTCATTACTGATCCGACGTATTGATCA
GTTTGTGGCCGCGCATCCTGAATATGGAAGCCGTTCGGGCTTCCTTTCTCGCGTCGCTGCTGACAAGGTTATTAATCAAC
GATAG

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4