Detailed information of TA system TA123860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1793589..1794205
• Replicon: chromosome
• Accession: NZ_CP039388
• Organism: Enterobacter hormaechei strain cre64

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EZJ11_RS08655
• Protein ID: WP_015569913.1
• Coordinates: 1793834..1794205 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: EZJ11_RS08650
• Protein ID: WP_015569912.1
• Coordinates: 1793589..1793831 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZJ11_RS08620 (EZJ11_08775)	1788648..1789448	+	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-
EZJ11_RS08625 (EZJ11_08780)	1789567..1790166	+	600	WP_003861947.1	glucose-1-phosphatase	-
EZJ11_RS08630 (EZJ11_08785)	1790160..1791041	+	882	WP_003861949.1	virulence factor BrkB family protein	-
EZJ11_RS08635 (EZJ11_08790)	1791038..1791475	+	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
EZJ11_RS08640 (EZJ11_08795)	1791520..1792461	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
EZJ11_RS08645 (EZJ11_08800)	1792470..1793390	-	921	WP_015569911.1	alpha/beta hydrolase	-
EZJ11_RS08650 (EZJ11_08805)	1793589..1793831	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
EZJ11_RS08655 (EZJ11_08810)	1793834..1794205	+	372	WP_015569913.1	PIN domain-containing protein	Toxin
EZJ11_RS08660 (EZJ11_08815)	1794245..1795174	-	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
EZJ11_RS08665 (EZJ11_08820)	1795171..1795806	-	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
EZJ11_RS08670 (EZJ11_08825)	1795803..1796705	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T123860 WP_015569913.1 NZ_CP039388:1793834-1794205 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T123860 NZ_CP039388:1793834-1794205 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGAGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT123860 WP_015569912.1 NZ_CP039388:1793589-1793831 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT123860 NZ_CP039388:1793589-1793831 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3