Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 487148..487785 Replicon chromosome
Accession NZ_CP039380
Organism Bacillus velezensis strain LPL-K103

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag SB21_RS02500 Protein ID WP_003156187.1
Coordinates 487435..487785 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag SB21_RS02495 Protein ID WP_003156188.1
Coordinates 487148..487429 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SB21_RS02475 483513..484112 - 600 WP_020953896.1 rhomboid family intramembrane serine protease -
SB21_RS02480 484205..484570 + 366 WP_007410229.1 holo-ACP synthase -
SB21_RS02485 484735..485742 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
SB21_RS02490 485859..487028 + 1170 WP_039062648.1 alanine racemase -
SB21_RS02495 487148..487429 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
SB21_RS02500 487435..487785 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
SB21_RS02505 487903..488724 + 822 WP_003156182.1 STAS domain-containing protein -
SB21_RS02510 488729..489094 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
SB21_RS02515 489097..489498 + 402 WP_003156178.1 anti-sigma regulatory factor -
SB21_RS02520 489510..490517 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
SB21_RS02525 490581..490910 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
SB21_RS02530 490907..491389 + 483 WP_003156173.1 anti-sigma B factor RsbW -
SB21_RS02535 491355..492143 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
SB21_RS02540 492143..492745 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T123852 WP_003156187.1 NZ_CP039380:487435-487785 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T123852 NZ_CP039380:487435-487785 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT123852 WP_003156188.1 NZ_CP039380:487148-487429 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT123852 NZ_CP039380:487148-487429 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References