Detailed information of TA system TA123852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 487148..487785
• Replicon: chromosome
• Accession: NZ_CP039380
• Organism: Bacillus velezensis strain LPL-K103

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: SB21_RS02500
• Protein ID: WP_003156187.1
• Coordinates: 487435..487785 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: SB21_RS02495
• Protein ID: WP_003156188.1
• Coordinates: 487148..487429 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SB21_RS02475	483513..484112	-	600	WP_020953896.1	rhomboid family intramembrane serine protease	-
SB21_RS02480	484205..484570	+	366	WP_007410229.1	holo-ACP synthase	-
SB21_RS02485	484735..485742	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
SB21_RS02490	485859..487028	+	1170	WP_039062648.1	alanine racemase	-
SB21_RS02495	487148..487429	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
SB21_RS02500	487435..487785	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
SB21_RS02505	487903..488724	+	822	WP_003156182.1	STAS domain-containing protein	-
SB21_RS02510	488729..489094	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
SB21_RS02515	489097..489498	+	402	WP_003156178.1	anti-sigma regulatory factor	-
SB21_RS02520	489510..490517	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
SB21_RS02525	490581..490910	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
SB21_RS02530	490907..491389	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
SB21_RS02535	491355..492143	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
SB21_RS02540	492143..492745	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T123852 WP_003156187.1 NZ_CP039380:487435-487785 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T123852 NZ_CP039380:487435-487785 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT123852 WP_003156188.1 NZ_CP039380:487148-487429 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT123852 NZ_CP039380:487148-487429 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5