Detailed information of TA system TA123849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 3341763..3342378
• Replicon: chromosome
• Accession: NZ_CP039374
• Organism: Halomonas titanicae strain ANRCS81

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1G8NTB1
• Locus tag: E8A47_RS15285
• Protein ID: WP_022522690.1
• Coordinates: 3341763..3342059 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E8A47_RS15290
• Protein ID: WP_022522689.1
• Coordinates: 3342064..3342378 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8A47_RS15265	3337467..3337982	-	516	WP_022522693.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
E8A47_RS15270	3338202..3338474	+	273	WP_008958942.1	XRE family transcriptional regulator	-
E8A47_RS15275	3338602..3339795	-	1194	WP_200890240.1	chromate efflux transporter	-
E8A47_RS15280	3339908..3341590	-	1683	WP_009287633.1	dihydroxy-acid dehydratase	-
E8A47_RS15285	3341763..3342059	+	297	WP_022522690.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E8A47_RS15290	3342064..3342378	+	315	WP_022522689.1	putative addiction module antidote protein	Antitoxin
E8A47_RS15295	3342525..3346154	+	3630	WP_141440875.1	exodeoxyribonuclease V subunit gamma	-
E8A47_RS15300	3346163..3347098	-	936	WP_041158175.1	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11045.95 Da
• Isoelectric Point: 10.2250

>T123849 WP_022522690.1 NZ_CP039374:3341763-3342059 [Halomonas titanicae]
MIELIKTDVFDRWLRELRDIRARAKVEARIRRLSLGNPGDVKPVGKGVSELRIPYGPGYRVYYITKGPVIVVLLCGGDKA
TQSHDIELAKAIAAQWKE

Nucleotide

• Length: 297 bp

>T123849 NZ_CP039374:3341763-3342059 [Halomonas titanicae]
ATGATCGAGTTGATTAAAACCGATGTCTTCGATAGATGGCTACGCGAGCTTCGCGATATCCGTGCCCGCGCCAAGGTTGA
AGCCCGCATCCGACGTTTGAGTCTGGGCAACCCTGGAGATGTGAAGCCGGTCGGTAAAGGCGTTTCTGAACTGCGAATTC
CTTATGGGCCAGGCTACCGCGTCTATTATATTACCAAGGGGCCAGTAATCGTGGTTCTGCTGTGCGGTGGTGATAAAGCC
ACCCAATCCCACGATATCGAACTAGCCAAAGCCATTGCCGCGCAATGGAAGGAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11074.48 Da
• Isoelectric Point: 4.4217

>AT123849 WP_022522689.1 NZ_CP039374:3342064-3342378 [Halomonas titanicae]
MTVPNVTFSHYDTADYLQTEEDIAAYLDAVMEENDPALLAAALGDIARARNMSQLAKDVGMSREGLYKALSGEGNPAFAT
ISKVANSLGLRLSIKPLSRPEAGS

Nucleotide

• Length: 315 bp

>AT123849 NZ_CP039374:3342064-3342378 [Halomonas titanicae]
ATGACCGTTCCTAATGTCACTTTCAGTCACTACGATACTGCTGATTATCTTCAAACTGAGGAGGATATTGCGGCCTATCT
TGATGCAGTAATGGAAGAAAATGATCCTGCTCTTCTTGCCGCCGCACTTGGTGATATCGCCCGTGCTCGCAACATGAGCC
AACTCGCCAAAGACGTAGGCATGAGCCGTGAAGGGCTTTACAAAGCCCTATCGGGGGAAGGAAACCCGGCTTTTGCGACC
ATTAGCAAAGTAGCCAACTCCTTGGGGTTGCGCCTATCTATTAAACCGCTATCGCGGCCAGAAGCGGGCAGCTAG

Structures

Toxin

• AlphaFold DB: A0A1G8NTB1