Detailed information of TA system TA123827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 870119..870729
• Replicon: plasmid pUW386
• Accession: NZ_CP039340
• Organism: Ralstonia solanacearum strain UW386

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0S4TTR7
• Locus tag: E7Z57_RS20835
• Protein ID: WP_064050709.1
• Coordinates: 870119..870427 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E7Z57_RS20840
• Protein ID: WP_016722339.1
• Coordinates: 870424..870729 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7Z57_RS20815	866460..867056	-	597	WP_138930096.1	GNAT family N-acetyltransferase	-
E7Z57_RS20820	867189..867740	+	552	WP_064050712.1	helix-turn-helix transcriptional regulator	-
E7Z57_RS20825	867903..868718	+	816	WP_064050711.1	GntR family transcriptional regulator	-
E7Z57_RS20830	868845..870044	+	1200	WP_064050710.1	MFS transporter	-
E7Z57_RS20835	870119..870427	+	309	WP_064050709.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E7Z57_RS20840	870424..870729	+	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
E7Z57_RS20845	870942..872255	+	1314	WP_064050708.1	metabolite/H+ symporter	-
E7Z57_RS20850	872359..873027	-	669	WP_081262000.1	hypothetical protein	-
E7Z57_RS20860	873693..873977	+	285	WP_064050707.1	hypothetical protein	-
E7Z57_RS20865	874109..874390	+	282	WP_138930097.1	hypothetical protein	-
E7Z57_RS20870	874706..875098	-	393	WP_064050705.1	RidA family protein	-
E7Z57_RS20875	875126..875536	-	411	WP_011003359.1	acyl-CoA thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / fliI / fliM / flgG / flgC / cyaB / motA / cheW / cheB / cheY / adeG	1..1830097	1830097

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11684.35 Da
• Isoelectric Point: 6.8614

>T123827 WP_064050709.1 NZ_CP039340:870119-870427 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDTAAQRRIQVRIDRLQMGNPGDMKAVRDGIHGLRIDHGPGHRLYVVQHGVVLIVLLCGGDKS
TQETDIRRAIDQSRRLDVDDME

Nucleotide

• Length: 309 bp

>T123827 NZ_CP039340:870119-870427 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACACGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCAACCCGGGCGACATGAAAGCGGTCCGTGACGGCATCCATGGGCTGCGGATCG
ATCATGGGCCGGGCCACCGCTTGTACGTCGTGCAGCATGGCGTGGTCCTGATCGTGCTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCAGTCCCGCCGCCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT123827 WP_016722339.1 NZ_CP039340:870424-870729 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT123827 NZ_CP039340:870424-870729 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGACGACCCCGCCTTCATCACGCACGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACCGGGATGACCCGCGAAGGACTGTACAAAGCGCTTTCCGAGGGCGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A0S4TTR7