Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 2816227..2816888 Replicon chromosome
Accession NZ_CP039339
Organism Ralstonia solanacearum strain UW386

Toxin (Protein)


Gene name vapC Uniprot ID A0A399W071
Locus tag E7Z57_RS13370 Protein ID WP_003278076.1
Coordinates 2816475..2816888 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A5B7U6Y4
Locus tag E7Z57_RS13365 Protein ID WP_064051996.1
Coordinates 2816227..2816478 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E7Z57_RS13355 2811651..2812046 - 396 WP_138929303.1 hypothetical protein -
E7Z57_RS13360 2812052..2816152 - 4101 WP_138929304.1 phage tail tape measure protein -
E7Z57_RS13365 2816227..2816478 + 252 WP_064051996.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
E7Z57_RS13370 2816475..2816888 + 414 WP_003278076.1 type II toxin-antitoxin system VapC family toxin Toxin
E7Z57_RS13380 2817820..2818620 + 801 WP_138929305.1 hypothetical protein -
E7Z57_RS13385 2819553..2819843 + 291 WP_064049985.1 H-NS histone family protein -
E7Z57_RS13390 2819886..2820530 - 645 WP_138928269.1 hypothetical protein -
E7Z57_RS13395 2820505..2820726 - 222 WP_138929306.1 hypothetical protein -
E7Z57_RS13400 2820723..2821112 - 390 WP_138929307.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2801785..2853621 51836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-63)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15756.12 Da        Isoelectric Point: 6.2337

>T123825 WP_003278076.1 NZ_CP039339:2816475-2816888 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKQPDARVLAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLETELPNYFLGRLLDID
AHTADRWGRLMSSANRPLPAIDGLLAATALQHDLILVTRNTKDFVGLDVPLINPWET

Download         Length: 414 bp

>T123825 NZ_CP039339:2816475-2816888 [Ralstonia solanacearum]
TTGAGCTACCTGATCGACACCAATGTGCTGTCCGAGCTACGCCGCAAGCAGCCCGATGCGCGCGTGCTCGCCTGGATGCA
AGACCGGCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAAATCCGCAAAGGCATCGAACGGCTGGACGACG
CCGCACGCCGCCAGCACCTGATCGACTGGCTCGAAACAGAATTGCCGAACTACTTCCTCGGCCGCTTGCTCGACATCGAT
GCCCATACCGCCGACCGCTGGGGCCGGCTGATGTCGAGCGCCAACCGGCCGCTGCCGGCCATCGATGGCCTGCTCGCCGC
GACCGCCCTGCAGCACGACCTCATACTGGTCACGCGCAACACGAAGGATTTCGTCGGACTCGATGTCCCGCTCATCAACC
CTTGGGAAACGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9272.49 Da        Isoelectric Point: 5.0774

>AT123825 WP_064051996.1 NZ_CP039339:2816227-2816478 [Ralstonia solanacearum]
MQSWQLQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGSGESLVDFMRQSPLAGLDDVVFERERSLPRE
VEF

Download         Length: 252 bp

>AT123825 NZ_CP039339:2816227-2816478 [Ralstonia solanacearum]
ATGCAAAGCTGGCAGTTGCAAGCCGCCAAGGCGCGGTTTTCCGACGTCGTGAAGCGGGCAGCGGACGATGGTCCGCAGGA
AATCACGGTGCATGGCCGCCCGGTTGCGGTTGTGATCTCACGCGCACTGTTCGATCGCTTGAGTGGCAGCGGGGAGTCGC
TGGTCGACTTCATGCGCCAGTCTCCGTTGGCTGGCCTGGACGATGTCGTCTTCGAGCGCGAGCGCAGCCTGCCGCGCGAG
GTCGAGTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A399W071

Antitoxin

Source ID Structure
AlphaFold DB A0A5B7U6Y4

References