Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 2620824..2621457 Replicon chromosome
Accession NZ_CP039339
Organism Ralstonia solanacearum strain UW386

Toxin (Protein)


Gene name vapC Uniprot ID A0A0S4TXD2
Locus tag E7Z57_RS12420 Protein ID WP_064048317.1
Coordinates 2620824..2621237 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID D8N529
Locus tag E7Z57_RS12425 Protein ID WP_020747258.1
Coordinates 2621224..2621457 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E7Z57_RS12400 2616546..2618123 + 1578 WP_064048320.1 glycerol-3-phosphate dehydrogenase -
E7Z57_RS24755 2618167..2618289 + 123 Protein_2384 ABC transporter -
E7Z57_RS12405 2618290..2618517 + 228 Protein_2385 carbohydrate ABC transporter substrate-binding protein -
E7Z57_RS12410 2618679..2620175 + 1497 WP_064048318.1 glycerol kinase GlpK -
E7Z57_RS12415 2620220..2620849 + 630 WP_064048323.1 DUF1415 family protein -
E7Z57_RS12420 2620824..2621237 - 414 WP_064048317.1 PIN domain-containing protein Toxin
E7Z57_RS12425 2621224..2621457 - 234 WP_020747258.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E7Z57_RS12430 2621514..2623121 - 1608 WP_064048316.1 glucan biosynthesis protein -
E7Z57_RS12435 2623334..2624566 + 1233 WP_138929198.1 AGE family epimerase/isomerase -
E7Z57_RS12440 2624574..2625869 + 1296 WP_138929199.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-118)

Antitoxin

SpoVT-AbrB domain

(5-39)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15165.41 Da        Isoelectric Point: 5.5348

>T123824 WP_064048317.1 NZ_CP039339:c2621237-2620824 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADTAETLLMAGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRLAERYGWSVYDALIVAAALLAGCNVLYSEDMQHGQVIEQHLRIVNPFSARA

Download         Length: 414 bp

>T123824 NZ_CP039339:c2621237-2620824 [Ralstonia solanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATACGGC
GGAAACGCTGCTGATGGCCGGCGGTGTCGTCAGCGTACAGGTGCTGAACGAAACGACGCACGTGATGCGCCGCAAGCTCG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAAGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTCTAGCAGAACGCTACGGATGGTCGGTCTATGACGCGCTGATCGTCGCCGCGGCGTTGCTTGC
CGGCTGCAACGTGCTGTATTCCGAAGACATGCAGCACGGCCAGGTGATTGAGCAGCATCTGCGCATCGTCAATCCTTTTT
CGGCACGGGCATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8806.98 Da        Isoelectric Point: 8.7287

>AT123824 WP_020747258.1 NZ_CP039339:c2621457-2621224 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRSARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT123824 NZ_CP039339:c2621457-2621224 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCCATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAACTGCACGAGGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGGGTCCGCCGCAAGCGCTCCGCGCGCGAGCTGTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S4TXD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0S4TW23

References