Detailed information of TA system TA123823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 2016698..2017395
• Replicon: chromosome
• Accession: NZ_CP039339
• Organism: Ralstonia solanacearum strain UW386

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: E7Z57_RS09755
• Protein ID: WP_138928989.1
• Coordinates: 2016698..2017057 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: E7Z57_RS09760
• Protein ID: WP_138928990.1
• Coordinates: 2017054..2017395 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7Z57_RS09740	2012000..2012512	-	513	WP_138928987.1	helix-turn-helix domain-containing protein	-
E7Z57_RS09745	2012548..2015543	+	2996	Protein_1879	toprim domain-containing protein	-
E7Z57_RS09750	2015547..2016638	+	1092	WP_138928988.1	site-specific tyrosine recombinase XerC	-
E7Z57_RS09755	2016698..2017057	+	360	WP_138928989.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E7Z57_RS09760	2017054..2017395	+	342	WP_138928990.1	helix-turn-helix domain-containing protein	Antitoxin
E7Z57_RS24725	2017402..2017554	+	153	WP_175425993.1	hypothetical protein	-
E7Z57_RS09765	2017541..2017738	+	198	WP_138928991.1	hypothetical protein	-
E7Z57_RS09770	2017974..2019455	+	1482	WP_138928992.1	HNH endonuclease	-
E7Z57_RS09775	2019458..2019937	+	480	WP_138928993.1	hypothetical protein	-
E7Z57_RS09780	2020260..2020463	+	204	WP_138928994.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-94)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12978.91 Da
• Isoelectric Point: 9.6106

>T123823 WP_138928989.1 NZ_CP039339:2016698-2017057 [Ralstonia solanacearum]
MPTIRPLRWVGSAKKDLGSMPADVQDTFGYALHLAQVGGKHSQAKPLKGYGGAGVLEVVEDHQGDTYRAVYTVRYAAAVY
VLHCFQKKSTHGIATPKPDMDLIDARLKAVAALEKERQT

Nucleotide

• Length: 360 bp

>T123823 NZ_CP039339:2016698-2017057 [Ralstonia solanacearum]
ATGCCGACGATCAGACCGCTGCGCTGGGTAGGTTCAGCCAAGAAAGACCTGGGTTCGATGCCCGCCGACGTGCAGGACAC
CTTCGGCTATGCGCTGCACCTGGCGCAGGTGGGTGGCAAGCACAGCCAGGCCAAGCCGCTCAAGGGCTACGGCGGGGCGG
GGGTGCTGGAAGTGGTGGAGGACCATCAGGGCGATACCTACCGCGCCGTGTACACGGTGCGCTATGCGGCGGCGGTGTAC
GTGCTGCATTGCTTCCAGAAGAAATCGACGCATGGGATTGCCACGCCCAAGCCGGACATGGACCTCATTGATGCCCGCCT
GAAGGCCGTGGCTGCATTGGAAAAGGAGCGCCAGACATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12420.43 Da
• Isoelectric Point: 9.8314

>AT123823 WP_138928990.1 NZ_CP039339:2017054-2017395 [Ralstonia solanacearum]
MNTPATIDDLVIEEGSTNPYADLGRPDADEMLVKAGLAHEIAQIIKRRHLTQQRAAELVGMPQSKLSELLRGKFRGISQA
KMIECLNRLGRDVDIVVKKAGRRALGHTHVVVA

Nucleotide

• Length: 342 bp

>AT123823 NZ_CP039339:2017054-2017395 [Ralstonia solanacearum]
ATGAACACGCCTGCAACCATCGATGACCTCGTGATCGAAGAGGGCAGCACCAACCCGTATGCCGACCTGGGCCGGCCGGA
CGCCGATGAAATGCTGGTGAAGGCAGGGCTGGCGCACGAGATCGCGCAGATCATTAAGCGCCGGCACCTGACGCAGCAGC
GCGCGGCCGAGTTGGTGGGGATGCCGCAGTCGAAGCTCTCGGAGTTGCTGCGCGGCAAGTTCCGGGGCATCAGCCAGGCC
AAGATGATCGAGTGCCTGAACCGCCTGGGCCGCGACGTGGACATCGTGGTGAAGAAAGCGGGCCGGCGCGCGCTGGGGCA
TACGCACGTCGTTGTTGCGTGA