Detailed information of TA system TA123821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 916643..917199
• Replicon: chromosome
• Accession: NZ_CP039339
• Organism: Ralstonia solanacearum strain UW386

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: E7Z57_RS04615
• Protein ID: WP_138928595.1
• Coordinates: 916969..917199 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0S4TUI1
• Locus tag: E7Z57_RS04610
• Protein ID: WP_064049835.1
• Coordinates: 916643..916954 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7Z57_RS04600	911684..913849	+	2166	WP_064049833.1	D-(-)-3-hydroxybutyrate oligomer hydrolase	-
E7Z57_RS04605	914010..916598	+	2589	WP_138928594.1	ATP-dependent chaperone ClpB	-
E7Z57_RS04610	916643..916954	-	312	WP_064049835.1	putative addiction module antidote protein	Antitoxin
E7Z57_RS04615	916969..917199	-	231	WP_138928595.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E7Z57_RS04620	917747..918757	+	1011	WP_138928596.1	sulfate ABC transporter substrate-binding protein	-
E7Z57_RS04625	918789..919433	+	645	WP_138928597.1	NADPH-dependent FMN reductase	-
E7Z57_RS04630	919569..920579	+	1011	WP_064049849.1	sulfonate ABC transporter substrate-binding protein	-
E7Z57_RS04635	920607..921770	+	1164	WP_138928598.1	FMNH2-dependent alkanesulfonate monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-73)

Antitoxin

• Cro/C1-type helix-turn-helix domain (51-95)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8308.78 Da
• Isoelectric Point: 11.7540

>T123821 WP_138928595.1 NZ_CP039339:c917199-916969 [Ralstonia solanacearum]
MRGVVVARIKRLERGLTGDVEPVGEGVSELRIHIGAGWRVYFTQRGAQLAVLLVGGSKRTQKRDIKRAKALAALLD

Nucleotide

• Length: 231 bp

>T123821 NZ_CP039339:c917199-916969 [Ralstonia solanacearum]
ATGCGCGGCGTGGTCGTGGCGAGGATCAAGCGCTTGGAACGCGGCCTGACGGGCGACGTTGAGCCGGTAGGCGAGGGCGT
CTCGGAGCTGCGCATCCACATCGGCGCGGGCTGGCGCGTGTACTTCACGCAGCGCGGCGCACAGCTGGCCGTGCTACTGG
TGGGCGGCTCCAAGCGCACGCAGAAGCGCGACATCAAGCGTGCCAAGGCGCTCGCGGCACTGCTGGACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11066.67 Da
• Isoelectric Point: 4.8231

>AT123821 WP_064049835.1 NZ_CP039339:c916954-916643 [Ralstonia solanacearum]
MTKQRIKVDELPEFDAAPYLDSEEAIAAYLTDILDTNDAGLLAAALGDIARARGMTEIAKAAGITREALYKALRPNSEPR
FDTVNRVCAALGVRLVAQAIHPA

Nucleotide

• Length: 312 bp

>AT123821 NZ_CP039339:c916954-916643 [Ralstonia solanacearum]
ATGACCAAGCAACGCATCAAGGTGGACGAACTGCCCGAGTTCGACGCCGCCCCGTACCTCGACAGCGAGGAAGCCATCGC
GGCCTACCTGACCGACATCCTGGACACGAACGACGCCGGCCTGCTGGCCGCCGCCCTGGGCGACATCGCCCGCGCTCGCG
GCATGACGGAAATCGCCAAGGCCGCCGGCATCACGCGGGAAGCCTTGTACAAGGCTCTGCGGCCAAACAGCGAGCCGCGC
TTCGACACCGTGAACCGCGTCTGCGCCGCCCTCGGCGTGCGTCTGGTCGCTCAGGCAATACACCCGGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S4TUI1